NEONATAL BRAIN STEM FUNCTION--CHEMICAL/PERFUSION AFFAIRS

新生儿脑干功能——化学/灌注事务

• 批准号: 3470183
• 负责人: MARLA R WOLFSON
• 金额: $ 10.04万
• 依托单位: TEMPLE UNIV OF THE COMMONWEALTH
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-08-01 至 1995-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3470183
• 关键词: acid base balance; artery; blood chemistry; brain stem; carbon dioxide tension; evoked potentials; hemodynamics; newborn animals; oxygen tension; perfusion; premature infant animal; sheep

The long term objective of the proposed research is to characterize and provide quantitative indices of the sensitivity of the neonatal brainstem evoked potential (BAEP) to alterations in arterial blood chemistry (ABC) and cerebral blood flow (CBF). The overall hypothesis of the proposed research is: Neonatal brainstem function sensitivity to alterations in ABC and CBF is age-dependent. This hypothesis will be tested as follows: The BAEP will be elicited, analyzed, and used as an index of brainstem function in very premature and full-term neonatal lambs. These animals will be studied at three experimentally controlled and independently varied levels of arterial carbon dioxide tension (PaCO(2)), pH, and CBF. Under each condition: 1) arterial oxygen tension (PaO(2)) will be held constant; 2) ABC and central hemodynamic status will be measured and/or calculated; 3) regional CBF, vascular resistance, and cerebral perfusion pressure will be measured and/or calculated. Utilizing this approach, the specific aims of the proposed research are to quantitatively determine the sensitivity of the neonatal BAEP to PaCO(2), pH, and CBF. These data will be further analyzed to identify a potential perfusion and age-dependent mechanism associated with PaCO(2), pH, or CBF induced alterations in the BAEP. This multidisciplinary study represents the first attempt to identify the sensitivity of neonatal brainstem function, and the BAEP in particular, to discrete and independent alterations in arterial blood chemistry and perfusion. Furthermore, it is particularly noteworthy that this study will incorporate techniques which will enable these factors to be studied over an age range previously limited to fetal animal preparations. As such the results should provide unique information regarding brainstem function at particularly vulnerable stages of development. Such information should be relevant to the clinical management of the increasing number of prematurely delivered infants and expand our understanding of maturational changes in CNS responsiveness at a basic science level.

拟议研究的长期目标是表征和 提供新生儿脑干灵敏度的定量指数 诱发了动脉血液化学改变的潜力（BAEP）（ABC） 和脑血流（CBF）。提议的总体假设 研究是：新生儿脑干功能对ABC改变的敏感性 CBF与年龄有关。该假设将进行如下检验： BAEP将被引起，分析和用作脑干索引 在非常过早和完整的新生儿羔羊中发挥作用。这些动物会 在三个实验控制和独立变化的情况下进行研究 动脉二氧化碳张力（PACO（2）），pH和CBF的水平。在下面 每个条件：1）动脉氧张力（PAO（2））将保持恒定； 2）将测量和/或计算ABC和中央血液动力学状态； 3）区域CBF，血管抵抗和脑灌注压力将 测量和/或计算。利用这种方法，具体目的 拟议的研究是定量确定的灵敏度 新生儿Baep到PACO（2），pH和CBF。这些数据将进一步 分析以确定潜在的灌注和年龄依赖性机制 与PACO（2），pH或CBF诱导的BAEP改变有关。 这项多学科研究代表了识别的首次尝试 新生儿脑干功能，尤其是BAEP的敏感性，对 动脉血液化学和独立改变和独立改变 灌注。此外，特别值得注意的是这项研究将 合并技术将使这些因素进行研究 以前的年龄范围仅限于胎儿动物制剂。这样 结果应提供有关脑干功能的独特信息 特别脆弱的发展阶段。这样的信息应该是 与过早数量增加的临床管理有关 送婴儿并扩展我们对成熟变化的理解 CNS在基础科学层面上的响应能力。