HUMAN ANTIBODY RESPONSIVENESS AND DENTAL CARIES

人类抗体反应与龋齿

• 批准号: 3220239
• 负责人: MARTIN LEVINE
• 金额: $ 8.96万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-07-01 至 1987-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3220239
• 关键词: Actinomyces; Streptococcus mutans; antibody specificity; dental caries; dental plaque; enzyme linked immunosorbent assay; gingivitis; histocompatibility antigens; human age group; immune response genes; immunoglobulin G; oral bacteria; secretory immune system

Studies in humans by the principal investigator have shown that precipitating serum antibodies to oral bacteria are associated with significant differences in dental caries severity and accumulation of bacterial plaque. One antibody precipitates with an epitope found on macromolecules from Actinomyces spp. and the other with the D-alanyl ester moeity of glycerol teichoic acids from Streptococci and Lactobacilli. Non-precipitating serum antibodies to the polyglycerol phosphate moeity of the latter antigen are not associated with caries severity. The D-alanyl ester epitope is stable at pH 5, but hydrolyses rapidly at physiologic pH. The major specific aim of this study is to quantitate the serum and salivary antibody response to each epitope by a sensitive enzymoimmunoassay in order to examine the relationships between epitope-specific local and systemic antibody responses, age, caries severity and plaque accumulation. A secondary aim is to elucidate a role for genetic constitution in response development. Serum IgG and secretory IgA originate from different modes of antigen exposure and respectively indicate systemic or local immune responses. The respective antigens, in culture filtrates or cell extracts of S. mutans GS-4 and A. viscosus ATCC 19246, will be coated on wells of microplates and the antibody content of serum or salivary samples determined. The amount of antibody bound in the absence or presence of appropriate haptens will be measured with alkaline phosphatase covalently conjugated to goat IgG specific for either human IgG or the secretory chain of human IgA. The difference in binding before and after inhibition will reflect epitope-specific antibody binding and will be quantified by comparison with an appropriate, strongly reactive serum. The relationship of the epitope-specific antibody binding contents to precipitin responses will be examined by multiple discriminant analysis. The interrelationship of the respective antibody contents to each other, age, caries severity, length of fluoride exposure, plaque accumulation and gingivitis will be examined by multiple regression analysis over the whole population and within various serum and saliva antibody response groups. Differences in caries severity or expression of HLA-A, B, C and DR specificities respectively by antibody response group will be examined by multiple discriminant analysis. The findings should identify new factors associated with caries protection and susceptibility.

主要研究者对人类的研究表明 沉淀口腔细菌的血清抗体与 龋齿严重程度和累积量存在显着差异 菌斑。 一种抗体与上发现的表位沉淀 来自放线菌属的大分子。另一个是D-丙氨酰酯 来自链球菌和乳杆菌的甘油磷壁酸部分。 聚甘油磷酸部分的非沉淀血清抗体 后一种抗原与龋齿严重程度无关。 D-丙氨酰 酯表位在 pH 5 时稳定，但在生理 pH 下迅速水解。 本研究的主要具体目的是定量血清和 通过灵敏的酶免疫分析检测唾液抗体对每个表位的反应 为了检查表位特异性局部和表位之间的关系 全身抗体反应、年龄、龋齿严重程度和牙菌斑积累。 第二个目标是阐明遗传构成在反应中的作用 发展。 血清 IgG 和分泌型 IgA 来源于不同的模式 抗原暴露 和 分别表明全身或局部免疫 回应。 培养滤液或细胞提取物中的相应抗原 S. mutans GS-4 和 A. viscous ATCC 19246，将包被在 微孔板和血清或唾液样本中的抗体含量 决定。 在不存在或存在的情况下结合的抗体量 适当的半抗原将用碱性磷酸酶共价测量 与对人 IgG 或分泌链具有特异性的山羊 IgG 缀合 人类 IgA。 抑制前后结合的差异将 反映表位特异性抗体结合，并通过以下方式量化 与适当的强反应性血清进行比较。 关系 表位特异性抗体结合内容对沉淀素反应的影响 将通过多重判别分析进行检查。 相互关系 各自的抗体含量、年龄、龋齿严重程度、 接触氟化物的时间长短、牙菌斑积聚和牙龈炎都会 通过对整个人群的多元回归分析进行检查 在各种血清和唾液抗体反应组中。 差异在于 龋齿严重程度或 HLA-A、B、C 和 DR 特异性的表达 分别由抗体反应组进行多个检查 判别分析。 研究结果应确定相关的新因素 具有防龋作用和易感性。