MICROBIAL ANTIGENS ASSOCIATED WITH SPECIFIC ADHERENCE

与特异性粘附相关的微生物抗原

• 批准号: 4692619
• 负责人: J O CISAR
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4692619
• 关键词: Actinomyces; Escherichia coli; Streptococcus; Streptococcus mutans; Streptococcus salivarius; Streptococcus sanguis; antibody formation; antibody specificity; bacterial antigens; cell aggregation; dental caries; erythrocytes; exo alpha sialidase; gingiva; gingivitis; human tissue; humoral immunity; hybridomas; immune complex; immunochemistry; membrane activity; microorganism immunology; monoclonal antibody; oral bacteria; periodontium disorder; surface antigens; tooth root

Studies are continuing to define the fimbrial adhesins of Actinomyces viscosus, an oral bacterium that colonizes tooth surfaces, forms dental plaque and contributes to the initiation of gingivitis and periodontal diseases. The type 1 fimbriae of actinomyces appear to be a principal adhesin for tooth surfaces as shown by studies of bacterial adsorption to saliva-treated hydroxyapatite. Recombinant DNA technology has been used to clone a structural gene that encodes the type 1 fimbrial subunit of A. viscosus strain T14V. This protein has a molecular weight of 62,000 daltons and is reactive with monospecific as well as monoclonal antibodies directed against actinomyces type 1 fimbriae. The type 2 fimbriae of actinomyces are antigenically and functionally distinct from type 1. Type 2 fimbriae account for the lactose-sensitive coaggregation of actinomyces with strains of Streptococcus sanguis and thus would be expected to promote interbacterial adherence within dental plaque. The gene encoding the type 2 fimbrial subunit of strain T14V has been cloned and the protein expressed in Eschedrichia coli has been partially purified. In addition to the study of type 2 fimbriae, immunochemical studies are continuing to define streptococcal carbohydrates that function as receptors for the actinomyces lectin. The receptor-associated polysaccharide of S. sanguis 34 has been purified and found to have N-acetylgalactosamine as the immunodominant group. These findings have begun to provide a structural basis for understanding the mechanisms of oral microbial adherence.

研究正在继续定义放线菌的菌毛粘附素 粘性细菌是一种口腔细菌，定植于牙齿表面，形成牙齿 菌斑并导致牙龈炎和牙周病的发生 疾病。 放线菌的 1 型菌毛似乎是主要的 细菌吸附研究表明牙齿表面的粘附素 唾液处理过的羟基磷灰石。 重组DNA技术已被用于 克隆编码 A 1 型菌毛亚基的结构基因。 粘性菌株T14V。 这种蛋白质的分子量为 62,000 道尔顿，可与单特异性抗体和单克隆抗体发生反应 针对 1 型放线菌菌毛。 2型菌毛 放线菌在抗原性和功能上与 1 型不同。 2 菌毛解释了放线菌对乳糖敏感的共聚集 血链球菌菌株，因此预计会促进 牙菌斑内细菌间的粘附。 编码类型的基因 T14V菌株2个菌毛亚基已克隆并表达蛋白 大肠杆菌中的部分已被纯化。 除了学习之外 对于 2 型菌毛，免疫化学研究正在继续定义 作为放线菌受体的链球菌碳水化合物 凝集素。 S. sanguis 34 的受体相关多糖已被 纯化并发现 N-乙酰半乳糖胺作为免疫优势 团体。 这些发现已开始为 了解口腔微生物粘附的机制。