ANTIGEN AND CYTOTOXIC ACTIVITY IN EIKENELLA CORRODENS

腐蚀艾克内拉的抗原和细胞毒性活性

• 批准号: 2132357
• 负责人: MARTIN LEVINE
• 金额: $ 3.65万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-03-01 至 1997-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2132357
• 关键词: Escherichia coli; Streptococcus sanguis; bacterial antigens; bacterial proteins; bacterial toxins; cytotoxicity; gene mutation; genetic library; genetic recombination; gram negative bacteria; intermolecular interaction; molecular cloning; monoclonal antibody; mutant; nucleic acid probes; oligonucleotides; oral bacteria; protein sequence; protein structure function; site directed mutagenesis; tissue /cell culture; transfection; transposon /insertion element

DESCRIPTION: This revised application describes experiments designed to clarify therole of a protein produced by E. corrodens which has been implicated as a toxin with effects on human epithelial cells, fibroblasts, and HL60 cells.A monoclonal antibody has been identified which blocks the toxic activities of the organism, and that Mab reacts with an approximately 72Kd molecule produced by E. corrodens. Interestingly, the Mab also cross-reacts with a protein of apparently 140Kd produced by S. sanguis. Antisera reactive to the 140Kd protein also react with the E. corrodens 72Kd molecule. The experimental plan described in this application is to clone and inactivate the gene encoding the 72Kd molecule from E. corrodens and evaluate the effects of its inactivation on the toxicity in various cell culture systems.

描述：这个修订后的申请描述了设计的实验 阐明腐蚀大肠杆菌产生的蛋白质的作用 已被认为是一种对人类上皮细胞有影响的毒素， 成纤维细胞和 HL60 细胞。已鉴定出单克隆抗体 它可以阻止生物体的毒性活动，并且 Mab 会发生反应 具有由 E. corrodens 产生的大约 72Kd 分子。 有趣的是，单克隆抗体还与一种明显的蛋白质发生交叉反应。 140Kd 由血链球菌产生。 对 140Kd 蛋白有反应的抗血清 也与 E. Corrodens 72Kd 分子发生反应。 实验的 本申请中描述的计划是克隆并灭活该基因 编码 E. corrodens 的 72Kd 分子并评估效果 其失活对各种细胞培养系统中毒性的影响。