SCHIOTOSOME-SNAIL COMPATIBILITY: UNDERLYING MECHANISMS

裂殖体与蜗牛的相容性：潜在机制

• 批准号: 3444511
• 负责人: CHRISTOPHER JEFFREY BAYNE
• 金额: $ 14.03万
• 依托单位: OREGON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-09-30 至 1986-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3444511
• 关键词: cell cell interaction; cellular immunity; communicable disease transmission; host organism interaction; immunochemistry; membrane activity; microorganism immunology; protozoal antigen; schistosomiasis; tissue /cell culture

This research is aimed at providing an understanding of the mechanisms used by Schistosoma mansoni to survive and multiply in its intermediate host snail Biomphalaria glabrata. S. mansoni is a causative agent of human schistosomiasis, a serious debilitating disease in human populations in many areas of the tropics. On the premise that the compatibility of S. mansoni with B. glabrata is a highly specific phenomenon, and that the specificity is manifested as a result of events occurring at the interface of the sporocyst with host-snail hemocyte plasma membranes, we aim to provide information on the cellular-chemical basis of specificity. Experiments will be designed to yield information which will support or refute various hypotheses that bear directly on the following issues: (a) The mechanisms by which snail hemocytes recognize trematode sporocysts (b) The mechanisms by which snails acquire enhanced resistance, of a more or less specific nature, to trematode sporocysts (c) The mechanisms by which trematode sporocysts avoid destruction by hemocytes of certain snail strains (d) The mechanisms by which trematode sporocysts interfere with innate resistance of snails Methodologies will involve in vitro culture of schistosome sporocyst larvae together with immunocompetent leukocytes (hemocytes) of host snails. Both susceptible and resistant strains of snail will be used as sources of hemocytes. Sensitive labeling of potentially important molecules of host and parasite origin will permit us to evaluate their roles in mediation of compatibility.

这项研究旨在提供对所使用机制的理解 由曼氏血吸虫属于其中间宿主的生存和繁殖 蜗牛生物掌labrata。 S. Mansoni是人类的病因 血吸虫病，一种严重的人类人群中的严重使人衰弱的疾病 热带地区的许多领域。 在以下前提是S.的兼容性。 曼森（Mansoni 特异性是由于接口处发生的事件而表现出来的 带有宿主 - 螺旋血细胞质膜的孢子囊的孢子体 提供有关特异性细胞化学基础的信息。 实验将旨在产生支持或 驳斥直接在以下问题上直接存在的各种假设： （a）蜗牛血细胞识别巨囊的机制 （b）蜗牛获得增强的阻力的机制， 或不太具体的性质 （c）trematode孢子囊避免破坏的机制 某些蜗牛菌株的血细胞 （d）trematode孢子囊干扰先天的机制 蜗牛的抗性 方法论将涉及黑素孢子菌幼虫的体外培养 以及宿主蜗牛的免疫能力白细胞（血细胞）。 两个都 蜗牛的敏感和抗性菌株将被用作来源 血细胞。 宿主潜在重要分子的敏感标记 寄生虫起源将使我们能够评估他们在调解中的作用 兼容性。