SCHISTOSOME-SNAIL COMPATIBILITY: UNDERLYING MECHANISMS

血吸虫与蜗牛的相容性：潜在机制

• 批准号: 3566937
• 负责人: CHRISTOPHER JEFFREY BAYNE
• 金额: $ 15.43万
• 依托单位: OREGON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-09-30 至 1992-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3566937
• 关键词: Gastropoda; Schistosoma mansoni; Trematoda; antigen receptors; cell cell interaction; cellular immunity; communicable disease transmission; gel electrophoresis; host organism interaction; humoral immunity; immunochemistry; immunofluorescence technique; laboratory mouse; laboratory rabbit; membrane activity; microorganism immunology; protozoal antigen; radiotracer; schistosomiasis; tissue /cell culture

Broad objectives of our research are to understand the cellular and molecular mechanisms which result in transmission of the human blood fluke Schistosoma mansoni in certain individual host snails (Biomphalaria glabrata), and the failure of transmission in others. This requires knowledge of the events which culminate in compatibility and those that result in incompatibility. Specifically we seek to identify parasite-borne characters which modulate infectivity and host-borne characters which determine susceptibility or resistance. In order to describe molecular events involved in parasite recognition by the intermediate host snail, the complex sporocyst- hemocyte system which occurs in vitro will be simplified for experimental analysis. Sporocyst surface components will be placed on activated beads. Hemocyte recognition of these sporocyst- labelled beads will be assessed by association of the two in vitro. Association indices will be obtained for hemocytes from snails which are susceptible to the schistosome and those from snails which are resistant. Hemocytes from naive and from parasite- exposed snails will be compared. The influence of snail plasma will be evaluated in experiments where the sporocyst-labelled beads are exposed to plasma before they are offered to hemocytes. Plasma will be derived from both susceptible and resistant snail strains, and from naive parasite-exposed individuals. Recognition factors on hemocyte surfaces and those from the plasma will be studied for their influence on the outcome of hemocyte- sporocyst encounters using a well tried cytotoxicity assay system.

我们研究的广泛目标是了解细胞和 导致人传播的分子机制 某些单个宿主蜗牛中的血液fluke fluke schistosoma mansoni （Biomphalaria glabrata），以及其他传播的失败。 这需要了解最终的事件 兼容性以及导致不兼容的兼容性。 具体而言，我们试图确定寄生虫传播的角色 调节感染力和宿主传播角色的确定 敏感性或抵抗力。 为了描述寄生虫涉及的分子事件 中间宿主蜗牛的识别，复杂的孢子囊 体外发生的血细胞系统将被简化 实验分析。 将放置孢子囊表面成分 在激活的珠子上。 这些孢子细胞的血细胞识别 标记的珠将通过两个体外的关联来评估。 将从蜗牛中获得有关血细胞的关联指数 容易受到该斑点的影响和蜗牛的 抗性。 来自幼稚和寄生虫的血细胞 将比较暴露的蜗牛。 蜗牛等离子体的影响 将在孢子标记珠的实验中评估 在将血浆送入血细胞之前，会暴露于血浆。 等离子体 将源自易感和抗性蜗牛菌株， 以及来自幼稚寄生虫的个体。 血细胞表面和血浆中的识别因子 将研究其对血细胞结果的影响 使用经过良好尝试的细胞毒性测定系统遇到孢子囊。