MOLECULAR BIOLOGY OF SCHISTOSOME-SNAIL INTERACTIONS

血吸虫-蜗牛相互作用的分子生物学

• 批准号: 3565492
• 负责人: WILLARD O GRANATH
• 金额: $ 6.5万
• 依托单位: UNIVERSITY OF MONTANA
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-07-01 至 1990-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3565492
• 关键词: Biomphalaria; Schistosoma; Schistosoma mansoni; antibody; autoradiography; blood cells; cytotoxicity; disease vectors; gel electrophoresis; hemolymph; high performance liquid chromatography; host organism interaction; immunoregulation; laboratory mouse; laboratory rabbit; molecular biology; monoclonal antibody; radiotracer; schistosomiasis; surface antigens

The goal of this research is to apply modern technology to the study of schistosome-snail interactions. The techniques to be used include denaturing and nondenaturing slab gel electrophoresis, high-performance liquid chromatography (HPLC), 125I surface-labeling, and various immunological techniques (immunoblotting, monoclonal and polyclonal antibody production, etc.). Such methods either have not been applied or have not been systematically or extensively used to study the three major interacting components comprising the immune association between the vector snail, Biomphalaria glabrata, and Schistosoma mansoni. These techniques will be employed to examine circulating blood cells (hemocytes), cell-free hemolymph (serum), and the sporocyst tegumental surface in an effort to determine the molecular and biochemical parameters determining immune compatibility in this host-parasite system. This first will involve characterizing hemocyte membrane components of resistant (10- R2 strain) and susceptible (PR albino M-line strain) B. glabrata by polyacrylamide gel electrophoresis (PAGE), surface radioiodination, and immunoblotting. Serum also will be examined by PAGE, HPLC and immunoblotting. Next, the S. mansoni sporocyst tegument will be characterized by PAGE, radioiodination and immunoblotting. Finally, in vitro and in vivo experiments will be conducted to identify and functionally analyze surface antigens important in hemocyte-sporocyst binding and cytotoxicity, the significance of acquired host-snail antigens in parasite immune evasion, and important serum factors in parasite recognition and/or cytotoxicity. Results of such studies will provide valuable insights into how immune compatibility is manifested in schistosome-snail relationships. Moreover, the successful application of the proposed techniques will be extremely valuable in the analyses of other parasites and invertebrate vectors of human disease. Thus, the information gained from the proposed research will undoubtedly facilitate how we deal with invertebrate vectors of human disease in the future.

这项研究的目的是将现代技术应用于 Schistosom-Snail相互作用的研究。 这项技术是 使用的包括剥落和非元素板凝胶 电泳，高性能液相色谱（HPLC）， 125i表面标记和各种免疫学技术 （免疫印迹，单克隆和多克隆抗体的产生， ETC。）。 此类方法尚未应用或没有使用过 系统或广泛地用于研究这三个主要 包括免疫关联的相互作用组件 在矢量蜗牛，生物掌和血吸虫之间 曼森。 这些技术将被用来检查 循环血细胞（血细胞），无细胞血淋巴（血清）， 以及孢子囊的表面，以确定 确定免疫的分子和生化参数 此主机寄生系统中的兼容性。 第一个将涉及 表征抗性的血细胞膜成分（10- R2菌株）和易感（PR白化M-Line菌株）B。glabrata通过 聚丙烯酰胺凝胶电泳（页面），表面 放射性化和免疫印迹。 还将检查血清 按页面，HPLC和免疫印迹。 接下来，S。Mansoni 孢子囊tegument将以页面为特征， 放射性化和免疫印迹。 最后，体外和体内 将进行实验以识别和功能 分析在血细胞 - 孢子结合中重要的表面抗原 和细胞毒性，获得的宿主钉抗原的意义 在寄生虫免疫逃避和重要的血清因子中 寄生虫识别和/或细胞毒性。 此类研究的结果 将为免疫兼容性的宝贵见解提供宝贵的见解 体现在棘体尺寸的关系中。 而且， 拟议技术的成功应用将是 在对其他寄生虫的分析中非常有价值 人类疾病的无脊椎动物载体。 因此，信息 从拟议的研究中获得的无疑将有助于 我们将来处理人类疾病的无脊椎动物媒介。