ELECTRON MICROSCOPE

电子显微镜

• 批准号: 3521057
• 负责人: WILLARD O GRANATH
• 金额: $ 20.6万
• 依托单位: UNIVERSITY OF MONTANA
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-04-25 至 1992-04-24
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3521057
• 关键词: biomedical equipment purchase; electron microscopy; ribonucleoproteins

The objective of this grant is to acquire a research quality transmission electron microscope (TEM) so that it can be used to perform a variety of research projects by PHS-supported investigators at the University of Montana (UM). Such studies can not be done currently since the extant instrument is obsolete and gives poor results. Presently, there are four PHS-supported investigators at. UM that would use the TEM to either pursue on-going research or to conduct experiments that are a logical extension of their NIH-supported research programs and include: 1. Immunoelectron microscopy and enzyme cytochemistry (at the EM level) will be used to examine immunobiological interactions between the human blood fluke. Schistosoma mansoni, and one of its' major intermediate hosts. Biomphalaria glabrata. 2. Immunoelectron microscopy and basic ultrastructural studies will be conducted to examine several aspects of the biology of Neisseria gonorrhoeae including topographic relationships of the outer membrane proteins, an examination of serum-sensitive and serum-resistant N. gonorrhoeae, and a characterization of naturally-elaborated outer membrane vesicles. 3. Immunoelectron microscopy, enzyme cytochemistry and autoradiography (at the EM level) will be used to conduct a detailed analysis of the effects of antivirals on early events in the replication of a feline AIDS virus. 4. Transmission electron microscopy will be used to examine the structures of ribonucleoprotein complexes of influenza virus. This will provide data needed to interpret results of functional analysis experiments to be conducted using scanning-tunnelling microscopy. Also, there are other PHS-supported investigators at UM that will need to use EM in the near future.

这笔赠款的目的是获得研究质量传输 电子显微镜（TEM），因此可以使用多种 PHS支持的研究人员的研究项目 蒙大拿州（UM）。 由于现存，目前无法进行此类研究 仪器已过时，并给出差的效果。 目前，有四个 PHS支持的调查人员在。嗯，那会用tem 进行正在进行的研究或进行合理的实验 扩展其NIH支持的研究计划，包括： 1。免疫电子显微镜和酶细胞化学（在EM水平上） 将用于检查人类之间的免疫生物学相互作用 流血。 曼氏菌和其主要中级之一 主持人。 Biomphalaria glabrata。 2。免疫电子显微镜和基本的超微结构研究将是 进行检查奈瑟氏菌生物学的几个方面 淋病，包括外膜的地形关系 蛋白质，对血清敏感和耐血清N的检查。 淋病，以及天然的外部表征 膜囊泡。 3。免疫电子显微镜，酶细胞化学和放射自显影 （在EM级别）将用于对 抗病人对猫艾滋病复制早期事件的影响 病毒。 4。透射电子显微镜将用于检查 流感病毒的核糖核蛋白复合物的结构。 这会 提供解释功能分析结果所需的数据 使用扫描调节显微镜进行的实验。 此外，UM还有其他PHS支持的调查员 在不久的将来使用EM。