CLIMBING FIBER TOPOGRAPHY ELICITED BY TACTILE STIMULI

触觉刺激引起的攀爬纤维形态

• 批准号: 3398296
• 负责人: LEE T ROBERTSON
• 金额: $ 11.36万
• 依托单位: GOOD SAMARITAN HOSP & MED CTR(PRTLND,OR)
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-04-01 至 1988-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3398296
• 关键词: body regions; cerebellar Purkinje cell; cerebellar cortex; cutaneous sensory nerve; electrophysiology; experimental brain lesion; histology; mathematical model; neural information processing; neuromuscular system; sensory feedback; sensory thresholds; stereotaxic techniques; stimulus /response; touch

The proposed research is a continuation of a long-term project to understand the organization in the cerebellar cortex of tactile information from the entire body surface as conveyed by the climbing fiber (CF) system. The proposed studies will examine the CF organization in several lobules of the cerebellum in cats (lobules VII to X, Crus I and II) and monkeys (lobule IV) anesthetized with sodium pentobarbital as well as in unanesthetized monkeys (lobules IV to VI). Multiple tungsten microelectrode penetrations will be made in each animal and extracellular CF responses will be recorded from individual Purkinje cells. Each response will be evaluated with natural tactile stimuli, and selected responses will be tested with a computer controlled mechanical stimulator. The main parameters to be examined will be: modality of the stimuli, force thresholds, and receptive field properties. Histological recostruction of the electrode penetrations will provide the localizaton of responses within the cortex. Acetylcholinesterase staining of cerebellar tissue will provide a comparison between the CF response properties and anatomically defined parasagittal zones. In monkeys prepared for chronic recording similar CF response properties will be studied. A comparison of the receptive field features will be made in the presence and absence of anesthetic. An analysis of receptive field features obtained from all the experiments will be used to develop a mathematical model of CF representation. An attempt will be made to determine how skin location information is encoded in different lobules and between different species. The proposed studies of the CF system will provide the first complete description of: (1) the spatial distributon of body surface representation throughout the cerebellar cortex, (2) the degree of cortical representation for various body surfaces, (3) the receptive field characteristics and the influence of an anesthetic on these characteristics, and (4) the extent of homogeneity in body surface representation for the anterior lobe between the cat and monkey. Such information is essential in order to understand what the CF system is encoding and how multiple representation of different body surfaces in various areas of the cerebellar cortex influences motor functions. These proposed studies will make an important contribution to the formulation of new theories of cerebellar function.

拟议的研究是一个长期项目的延续 了解触觉信息小脑皮层中的组织 从攀岩纤维（CF）传达的整个身体表面 系统。 拟议的研究将在几个中检查CF组织 小脑的小脑猫（小叶VII至x，crus I和ii）和 猴子（小叶IV）用果糖果糖和中的麻醉 未经麻醉的猴子（Lobules IV至VI）。 多个钨 微电极穿透将在每只动物和细胞外进行 CF响应将从单个Purkinje细胞中记录。 每个 响应将通过自然触觉刺激评估，并选择 响应将通过计算机控制的机械刺激器进行测试。 要检查的主要参数将是：刺激的模态，力 阈值和接受场特性。 组织学重建 电极穿透将提供响应的本地化 皮质。 小脑组织的乙酰胆碱酯酶染色将 提供CF响应属性与解剖学之间的比较 定义的副臂区域。 在为慢性记录准备的猴子中 将研究类似的CF响应属性。 比较 在存在和不存在的情况下将制作接受场特征 麻药。 对从所有的接受场特征的分析 实验将用于开发CF的数学模型 表示。 将尝试确定皮肤的位置 信息在不同的小叶中和不同物种之间编码。 CF系统的拟议研究将提供第一个完整 描述：（1）身体表面表示的空间分布 在整个小脑皮层中，（2）皮质表示程度 对于各种身体表面，（3）接受场特征和 麻醉对这些特征的影响，以及（4） 身体表面表示的同质性在前叶之间 猫和猴子。 这样的信息对于了解 CF系统的编码以及不同的多重表示 小脑皮层各个区域的身体表面会影响电动机 功能。 这些拟议的研究将为 小脑功能的新理论的表述。