NEURAL MECHANISMS OF CENTRAL CARDIOVASCULAR CONTROL

中枢心血管控制的神经机制

• 批准号: 3337516
• 负责人: JOHN B CABOT
• 金额: $ 12.94万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-01 至 1992-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3337516
• 关键词: autoradiography; axon; baroreceptors; blood pressure; central nervous system; dorsal raphe nucleus; electron microscopy; electrophysiology; histochemistry /cytochemistry; neural conduction; neural information processing; neural inhibition; neuroanatomy; neurogenic hypertension; pigeons; radiotracer; reflex; sympathetic ganglion; sympathetic nervous system

Three series of investigations are proposed. The first involves a comprehensive anatomical and physiological examination of the serotonergic raphe-SPN pathway. Using autoradiography, horseradish peroxidase (HRP), lesion, microstimulation, and extracellular recording techniques, studies have been designed to: (a) localize the cells of origin and spinal trajectory of the raphe-SPN system; (b) establish the conduction velocities and degree of convergence and divergence of raphe axons terminating upon the SPNs; (c) investigate the spatial and temporal properties of the inhibition of SPN discharge associated with activation of this descending system; (d) assess the involvement of the raphe in baroreceptor and somatosympathetic reflexes; and (e) establish the role of the raphe in the "tonic" and "phasic" modulation of arterial blood pressure. The second involves anterograde transport studies to localize the cells of origin of the catecholaminergic input(s) upon the SPNs. Using autoradiography, the spinal projections of cells in the ventrolateral medullary region, locus coeruleus, and subcoeruleus would be described. Anterograde tract tracing would also be initiated on the paraventricular nucleus of the hypothalamus. The final project involves a preliminary electron microscopic description of the cytological characteristics of axon terminals on HRP-labelled preganglionic neurons.

提出了三个系列调查。 第一个涉及全面 血清素能RAPHE-SPN途径的解剖学和生理检查。 使用放射自显影，辣根过氧化物酶（HRP），病变，微刺激， 和细胞外记录技术，研究旨在：（a） 定位Raphe-SPN系统的原点和脊柱轨迹； （b） 建立传导速度和收敛程度和差异 Raphe轴突终止于SPN； （c）研究空间和时间 与激活相关的SPN放电抑制的特性 下降系统； （d）评估raphe在压力感受器中的参与和 交感神经反射； （e）确定raphe在 动脉血压的“补品”和“阶段”调节。 第二个涉及顺行传输研究，以定位 SPN上的儿茶酚胺能输入的起源。 使用放射自显影， 腹外侧髓质区域的细胞的脊柱投影 将描述凝结和下层。 顺行跟踪将 也可以在下丘脑的室室核上启动。 最终项目涉及初步的电子显微镜描述 HRP标记的轴突末端的细胞学特征 神经元。