GERM CELL MIGRATION IN DROSOPHILA

果蝇生殖细胞迁移

• 批准号: 3308141
• 负责人: RAHUL WARRIOR
• 金额: $ 17.78万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-01 至 1998-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3308141
• 关键词: Drosophilidae; antiserum; cell cell interaction; cell differentiation; cell migration; complementary DNA; cytogenetics; embryo /fetus cell /tissue; gene complementation; gene expression; gene mutation; genetic mapping; genetic transcription; gonads; histogenesis; in situ hybridization; northern blottings; nucleic acid sequence; phenotype; polymerase chain reaction; transposon /insertion element; western blottings

The long term objective of this research is to learn how groups of cells at different locations in an embryo interact with each other to form a complex structure. This proposal outlines a study of the development and assembly of one such structure, the embryonic gonad of the fruitfly Drosophila . Understanding how the gonad is assembled will require learning more about the mechanism of cell specification, recognition, motility and cell-cell communication. Similar processes of cell specification and migration are characteristic of vertebrate and invertebrate systems both in normal development and in disease such as in the invasiveness and spread of metastatic tumors. This proposal has three specific aims: (I) To determine the role the Drosophila nudC gene plays in germ cell movement and gonad assembly. Drosophila nudC is a novel gene which is expressed in germ cells and is likely to play a role in nuclear or cell motility. The gene was identified using antisera from a patient with the autoimmune disease systemic lupus erythematosus. The gene encodes a protein 50% homologous to a protein required for nuclear movement in the fungus Aspergillus, the nudC gene. (II) To identify the positional cues necessary for pole cell movement and embryonic gonad formation. The assembly of the gonad can be separated into four separate steps each of which is mediated by distinct cues. This specific aim seeks to determine the positional cues required for germ cell movement and the assembly of the embryonic gonad. (III) To identify genes that are involved in the assembly of the gonad by collection of deficiency stocks, The third specific aim seeks to identify novel zygotic genes which play a role in germ cell movement and gonad assembly. This specific aim outlines a screen for zygotic genes using a collection of deletion stocks that cover about two thirds of the genome.

这项研究的长期目标是了解细胞组 在胚胎中的不同位置，彼此相互作用以形成 复杂的结构。 该提议概述了开发和 组装这样的结构，即果蝇的胚胎性腺 果蝇。了解性腺的组装方式将需要 了解有关细胞规范，识别，识别机制的更多信息 运动性和细胞电池通信。 类似的细胞过程 规范和迁移是脊椎动物的特征 正常发育和疾病中的无脊椎动物系统，例如 在转移性肿瘤的侵袭性和扩散中。 该提案具有三个具体目标： （i）确定果蝇NUDC基因在生殖细胞中的作用 运动和性腺组装。果蝇NUDC是一种新型基因 在生殖细胞中表达，可能在核或细胞中发挥作用 运动。 使用抗血清鉴定出该基因 自身免疫性疾病全身性红斑狼疮。 基因编码一个 蛋白质50％与核运动所需的蛋白质同源 NUDC基因真菌Aspergillus。 （ii）确定极细胞运动所需的位置提示和 胚胎性腺形成。性腺的组装可以分开 分为四个单独的步骤，每个步骤都由不同的提示介导。 该特定目的旨在确定 生殖细胞运动和胚胎性腺的组装。 （iii）确定与性腺组装有关的基因 通过收集不足库存，第三个特定目的试图 识别新型合子基因，这些基因在生殖细胞运动中起作用， 性腺大会。 该特定目的概述了二聚基因的屏幕 使用覆盖大约三分之二的删除库存的集合 基因组。