GERM CELL MIGRATION IN DROSOPHILA

果蝇生殖细胞迁移

基本信息

批准号：
2459473
负责人：
RAHUL WARRIOR
金额：
$ 19万
依托单位：
UNIVERSITY OF SOUTHERN CALIFORNIA
依托单位国家：
美国
项目类别：
财政年份：
1993
资助国家：
美国
起止时间：
1993-08-01 至 2000-07-31
项目状态：
已结题

项目摘要

The long term objective of this research is to learn how groups of cells at different locations in an embryo interact with each other to form a complex structure. This proposal outlines a study of the development and assembly of one such structure, the embryonic gonad of the fruitfly Drosophila . Understanding how the gonad is assembled will require learning more about the mechanism of cell specification, recognition, motility and cell-cell communication. Similar processes of cell specification and migration are characteristic of vertebrate and invertebrate systems both in normal development and in disease such as in the invasiveness and spread of metastatic tumors. This proposal has three specific aims: (I) To determine the role the Drosophila nudC gene plays in germ cell movement and gonad assembly. Drosophila nudC is a novel gene which is expressed in germ cells and is likely to play a role in nuclear or cell motility. The gene was identified using antisera from a patient with the autoimmune disease systemic lupus erythematosus. The gene encodes a protein 50% homologous to a protein required for nuclear movement in the fungus Aspergillus, the nudC gene. (II) To identify the positional cues necessary for pole cell movement and embryonic gonad formation. The assembly of the gonad can be separated into four separate steps each of which is mediated by distinct cues. This specific aim seeks to determine the positional cues required for germ cell movement and the assembly of the embryonic gonad. (III) To identify genes that are involved in the assembly of the gonad by collection of deficiency stocks, The third specific aim seeks to identify novel zygotic genes which play a role in germ cell movement and gonad assembly. This specific aim outlines a screen for zygotic genes using a collection of deletion stocks that cover about two thirds of the genome.

这项研究的长期目标是了解细胞群如何胚胎中的不同位置相互作用，形成结构复杂。该提案概述了对发展和果蝇胚胎性腺这样一种结构的组装果蝇。了解性腺如何组装需要了解更多关于细胞规范、识别的机制，运动和细胞间通讯。细胞的相似过程规格和迁移是脊椎动物的特征无脊椎动物系统在正常发育和疾病中，例如转移性肿瘤的侵袭和扩散。该提案有三个具体目标：（一）确定果蝇nudC基因在生殖细胞中的作用运动和性腺组装。果蝇 nudC 是一个新基因在生殖细胞中表达，可能在细胞核或细胞中发挥作用动力。该基因是使用患有该病的患者的抗血清鉴定出来的自身免疫性疾病系统性红斑狼疮。该基因编码一个与核运动所需蛋白质 50% 同源的蛋白质真菌曲霉菌，nudC 基因。 (II) 识别极细胞运动所需的位置线索胚胎性腺的形成。性腺的组装可以分离分成四个独立的步骤，每个步骤都由不同的线索介导。这一具体目标旨在确定所需的位置线索生殖细胞运动和胚胎性腺的组装。 (III) 鉴定参与性腺组装的基因通过收集缺陷库存，第三个具体目标旨在识别在生殖细胞运动中发挥作用的新合子基因性腺组装。这一具体目标概述了合子基因的筛选使用覆盖约三分之二的删除库存集合基因组。