Zeiss LSM 980 NLO Confocal Microscope with Airyscan2 for UCI Optical Biology Core

适用于 UCI 光学生物学核心的配备 Airyscan2 的 Zeiss LSM 980 NLO 共焦显微镜

• 批准号: 10429736
• 负责人: RAHUL WARRIOR
• 金额: $ 60万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-16 至 2023-09-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10429736
• 关键词: 10 year old; Affect; Aging; Antibiotics; Behavior; Biological Sciences; Biology; Biopolymers; Brain; California; Cells; Comprehensive Cancer Center; Contract Services; Data Analyses; Development; Disease; Engineering; Family; Frequencies; Funding; Grant; Health; Hour; Human; Image; Individual; Medicine; Memory; Microscope; Nerve Regeneration; Noise; Optics; Research; Research Personnel; Resolution; Resources; Sampling; Scanning; Schools; Signal Transduction; Structure; Synapses; Time; Training; Universities; Wages; base; cancer therapy; data acquisition; design; detector; fluorescence lifetime imaging; high resolution imaging; imaging capabilities; improved; instrument; instrumentation; macrophage; microscopic imaging; novel therapeutics; pandemic disease; physical science; response; tissue regeneration; two-photon; vector; wound healing

Project Summary/Abstract The Optical Biology Core (OBC) at the University of California, Irvine (UC Irvine) requests a Shared Instrumentation Grant to purchase a Zeiss LSM 980 Non-Linear Optics (NLO) confocal with Airyscan 2 FLIM ready. The LSM 980 will bring new capabilities such as higher resolution, more rapid data acquisition and AI- based sample finding that are not currently available to users. Importantly, the LSM 980 will replace a current microscope that is very heavily used and is over 10 years old and thus no longer eligible for service contract coverage after October 2022. The existing LSM 780 NLO confocal microscope has been used on average over 270 hours per month for the past 12 months, excluding the 3-month pandemic shutdown, with current usage close to 80 hours per week. This flagship instrument has been a critical component for researchers in over 34 labs across campus, and replacing it is essential to maintaining the highest level of instrument functionality, dependability, and availability across this wide disciplinary range. The LSM 980 will be located in dedicated space within the OBC. The OBC will provide administrative and technical support to maintain the microscope as well as advice on experimental set up, training and imaging, and data analysis expertise to enable users to take full advantage of its capabilities. The OBC has operated at UC Irvine since 1987 demonstrating its stability, capability and a long-standing institutional commitment to microscopy and imaging resources. The OBC receives robust financial commitments towards instrument service contracts, staff salaries and microscope accessories from a broad array of institutional sectors including the Office of Research, the NCI-funded Chao Family Comprehensive Cancer Center and four individual Schools: Biological Sciences, Engineering, Medicine, and Physical Sciences. Replacement of the existing LSM 780 will bring significantly improved imaging capabilities. The LSM 980 incorporates the Airyscan detector (first introduced in 2014), which samples high spatial frequencies with an end result of a two-fold improvement in resolution (120 nm and axial resolution of 350 nm) and up to an eight-fold improvement in signal to noise. Additional design and engineering improvements in the LSM 980 permit a larger field of view and much faster scanning than is possible with the LSM 780 while continuing to support two-photon and Fluorescence Lifetime Imaging (FLIM). These improved capabilities will enhance research currently using the LSM 780 by providing higher-resolution images in less time. Specific benefits to human health from projects of the major users include gaining a better understanding of: (1) how new drug therapies for cancer and other diseases can be enhanced; (2) delivery of synthetic vectors to cells; (3) how macrophages behave in response to natural and synthetic biopolymers; (4) antibiotic development; (5) tissue and nerve regeneration; (6) wound repair; and (7) how synaptic structures in the human brain are affected by behaviors, aging, and memory.

项目概要/摘要 加州大学欧文分校 (UC Irvine) 的光学生物学核心 (OBC) 请求共享 仪器补助金用于购买配备 Airyscan 2 FLIM 的 Zeiss LSM 980 非线性光学器件 (NLO) 共焦 准备好。 LSM 980将带来新的功能，例如更高分辨率、更快速的数据采集和AI- 基于当前无法向用户提供的样本发现。重要的是，LSM 980 将取代当前的 显微镜使用频繁且已使用超过 10 年，因此不再符合服务合同资格 2022 年 10 月后的覆盖范围。现有 LSM 780 NLO 共焦显微镜的平均使用时间超过 过去 12 个月每月 270 小时（不包括因疫情导致的 3 个月停工） 每周接近 80 小时。这款旗舰仪器已成为超过 34 个领域的研究人员的关键组件 整个校园的实验室，更换它对于维持最高水平的仪器功能至关重要， 在这个广泛的学科范围内的可靠性和可用性。 LSM 980 将位于 OBC 内的专用空间内。 OBC 将提供行政和 维护显微镜的技术支持以及实验设置、培训和成像方面的建议， 和数据分析专业知识，使用户能够充分利用其功能。 OBC 的运营时间为 加州大学欧文分校自 1987 年以来展示了其稳定性、能力和长期的机构承诺 显微镜和成像资源。 OBC 获得了对仪器服务的强有力的财务承诺 来自广泛机构部门的合同、员工工资和显微镜配件，包括 研究办公室、NCI 资助的 Chao 家族综合癌症中心和四所独立学校： 生物科学、工程、医学和物理科学。 替换现有的LSM 780将带来成像能力的显着提升。 LSM 980 结合了 Airyscan 检测器（于 2014 年首次推出），可对高空间频率进行采样 分辨率提高了两倍（120 nm，轴向分辨率为 350 nm），最高提高了八倍 信噪比的改善。 LSM 980 的额外设计和工程改进允许更大的 视野和比 LSM 780 更快的扫描速度，同时继续支持双光子 和荧光寿命成像 (FLIM)。这些改进的功能将增强目前使用的研究 LSM 780 可在更短的时间内提供更高分辨率的图像。项目对人类健康的具体好处 主要用户包括更好地了解：（1）新药如何治疗癌症和其他疾病 疾病可以得到加强； (2)将合成载体递送至细胞； (3) 巨噬细胞如何响应 天然和合成的生物聚合物； (4)抗生素开发； (5)组织和神经再生； (6)伤口 维修; (7) 人类大脑中的突触结构如何受到行为、衰老和记忆的影响。