ASSESSMENT OF MOUSE PREIMPLANTATION EMBRYOS

小鼠植入前胚胎的评估

• 批准号: 3320535
• 负责人: JOHN D BIGGERS
• 金额: $ 18.56万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-04-01 至 1992-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3320535
• 关键词: X ray spectrometry; acid base balance; aminoacid transport; antiport; bicarbonates; diagnosis design /evaluation; early embryonic stage; egg /ovum; embryo /fetus; embryo /fetus culture; embryo /fetus transplantation; fluorescent dye /probe; fluorimetry; glucose transport; glutamine; growth factor; growth media; hormone regulation /control mechanism; in vitro fertilization; insulin; insulinlike growth factor; laboratory mouse; mammalian embryology; meiosis; membrane permeability

The research proposed in this application is concerned with the intracellular pH (pHi) and metabolism of the oocyte and preimplantation stages of the mouse embryo. pH of the cells of single embryos will be measured by a method developed in our laboratories which uses one of the pH-sensitive fluorescent dyes. Detailed attention will be given to the absolute intracellular pH at different stages of development and its regulation. Specific attention will be paid to whether the pHi changes upon reinitiation of meiosis, fertilization, compaction, and/or exposure of the blastocyst to growth factors. The possibility of developing a non- invasive test of viability based on measurement of the acidification of the bathing medium when healthy embryos export protons to regulate their pHi will be investigated. The research is also concerned with the uptake of glutamine by single mouse preimplantation embryos. A new ultramicrofluorescence technique developed in our laboratories measures uptake of glutamine in the pmole range. The method will be used to (1) characterize the mechanisms that transport glutamine into and out of the preimplantation embryo, (2) study the action of insulin and related growth factors on the uptake of glutamine, and (3) investigate the possibility of measuring glutamine uptake by preimplantation embryos as a non-invasive test of viability.

本应用程序中提出的研究与 卵母细胞的细胞内pH（PHI）和代谢 小鼠胚胎的阶段。 单个胚胎细胞的pH将是 通过在我们的实验室中开发的方法来衡量，该方法使用了其中一种 pH敏感荧光染料。 将给予详细的关注 在发展的不同阶段的绝对细胞内pH及其 规定。 特别关注PHI是否改变 重新加入减数分裂，受精，压实和/或暴露 胚泡到生长因子。 开发非 - 的可能性 基于测量酸化的生存能力的侵入性测试 当健康的胚胎导出质子以调节其PHI时，沐浴培养基 将被调查。 该研究还涉及单小鼠对谷氨酰胺的摄取 植入前胚胎。 开发了一种新的超荧光技术 在我们的实验室中，测量PMOLE范围内谷氨酰胺的摄取。 这 方法将用于（1）表征运输机制 谷氨酰胺进入植入前胚胎，（2）研究作用 胰岛素和相关生长因子的谷氨酰胺摄取，（3） 研究测量谷氨酰胺摄取的可能性 植入前胚胎是一种无创的生存能力测试。

项目成果

Apparent absence of Na+/H+ antiport activity in the two-cell mouse embryo.

双细胞小鼠胚胎中明显缺乏 Na /H 反向转运活性。

• DOI: 10.1016/0012-1606(90)90208-z
• 发表时间: 1990
• 期刊: Developmental biology
• 影响因子: 2.7
• 作者: Baltz,JM;Biggers,JD;Lechene,C
• 通讯作者: Lechene,C

Intracellular ion concentrations and their maintenance by Na+/K(+)-ATPase in preimplantation mouse embryos.

植入前小鼠胚胎中细胞内离子浓度及其 Na /K( )-ATP 酶的维持。

• 发表时间: 1997
• 期刊: Zygote (Cambridge, England)
• 作者: Baltz,JM;Smith,SS;Biggers,JD;Lechene,C
• 通讯作者: Lechene,C

A novel H+ permeability dominating intracellular pH in the early mouse embryo.

一种新的 H 通透性主导早期小鼠胚胎中的细胞内 pH 值。

• DOI: 10.1242/dev.118.4.1353
• 发表时间: 1993
• 期刊: Development (Cambridge, England)
• 作者: Baltz,JM;Biggers,JD;Lechene,C
• 通讯作者: Lechene,C

On the number and rate of formation of sperm-zona bonds in the mouse.

关于小鼠精子-透明带键形成的数量和速率。

• DOI: 10.1002/mrd.1120240103
• 发表时间: 1989
• 期刊: Gamete research
• 作者: Baltz,JM;Cardullo,RA
• 通讯作者: Cardullo,RA

Noninvasive measurement of glucose uptake by two populations of murine embryos.

对两组小鼠胚胎的葡萄糖摄取进行无创测量。

• DOI: 10.1095/biolreprod39.4.779
• 发表时间: 1988
• 期刊: Biology of reproduction
• 影响因子: 3.6
• 作者: Butler,JE;Lechene,C;Biggers,JD
• 通讯作者: Biggers,JD