CULTURE MEDIA FOR PREIMPLANTATION DEVELOPMENT

植入前发育的培养基

• 批准号: 3552545
• 负责人: JOHN D BIGGERS
• 金额: $ 32.96万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-09-01 至 1991-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3552545
• 关键词: Ceboidea; Macaca mulatta; biological fluid transport; collagen; early embryonic stage; embryo /fetus culture; embryo /fetus transplantation; extracellular matrix; fibronectins; growth factor; growth media; in vitro fertilization; laboratory mouse; laboratory rabbit; laminin; mammalian embryology; membrane proteins; radioimmunoassay

The objective of the work is to design better techniques, particularly media, for in vitro fertilization and the culture of preimplantation embryos. The proposal is in two parts. The first part concerns the growth and differentiation of the mammalian blastocyst. Two animal models will be used, the mouse as a minimally expanding blastocyst, and the rabbit as a maximally expanding blastocyst. The response of the blastocysts to different media will be evaluated by measuring three major components of blastocyst development -- cell multiplication, the movement of fluid into the blastocyst and the synthesis of cell surface macromolecules. The initial phase will search for suitable available media that can provide the starting point for the addition of supplements such as amino acids and growth factors. Attempts will be made throughout to apply the principles of good experimental design to optimize the composition of media. The second part concerns finding suitable conditions for fertilizing primate oocytes in vitro. The purpose is to produce primate embryos for genetic manipulation, particularly to increase the numbers of genetically valuable animals for transfer, and by embryo bisection. The technique is also required for producing transgenic primates, which has importance in cancer research. Attempts will be made to increase the efficiency of in vitro fertilization in the rhesus monkey and the common marmoset.

工作的目的是设计更好的技术，尤其是 培养基，用于体外受精和植入前的培养 胚胎。 该提议分为两个部分。 第一部分涉及哺乳动物的生长和差异 胚泡。 将使用两种动物模型，将鼠标作为最小 扩展胚泡，兔子是最大扩展的胚泡。 胚泡对不同介质的响应将由 测量胚泡发育的三个主要组成部分 - 细胞 乘法，流体进入胚泡和合成 细胞表面大分子。 初始阶段将寻找合适的 可用的媒体可以为添加的起点提供起点 补充剂，例如氨基酸和生长因子。 将尝试 始终应用良好的实验设计原则以优化 媒体的组成。 第二部分涉及寻找适合施肥的条件 体外灵长类动物卵母细胞。 目的是生产灵长类动物胚胎 遗传操作，特别是为了增加基因的数量 有价值的动物转移和通过胚胎分配。 该技术是 生产转基因灵长类动物也需要 癌症研究。 尝试提高IN的效率 恒河猴和常见的果酱中的体外受精。