QUANTITATIVE TRAIT LOCI IN DROSOPHILA

果蝇数量性状位点

• 批准号: 3304495
• 负责人: TRUDY F. MACKAY
• 金额: $ 10.96万
• 依托单位: NORTH CAROLINA STATE UNIVERSITY RALEIGH
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-05-01 至 1995-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3304495
• 关键词: Drosophilidae; alleles; autosomal dominant trait; autosomal recessive trait; chromosomes; gene expression; gene mutation; genetic manipulation; genetic mapping; genetic polymorphism; genetic recombination; hair; in situ hybridization; molecular genetics; population genetics; southern blotting; statistics /biometry; transposon /insertion element

The long-term goal is to understand variation for quantitative traits in terms of the individual loci (QTLs) affecting them. Many inherited human diseases are quantitative traits. The specific aims are to determine whether alleles at loci with major morphological effects contribute to quantitative variation in natural populations, and to determine the extent and nature of the association between molecular and quantitative genetic variation at these loci. The model system to be used is the number of abdominal and sternopleural bristles in Drosophila melanogaster. Divergent artificial selection lines for sternopleural and abdominal bristle number will be started from a natural population and selected to the limit. The effects of the major chromosomes on selection response will be addressed. Chromosome substitution lines, in which a viable major chromosome of large effect from a high selection line is made homozygous and substituted into the homozygous low selection line background, will be made for each major chromosome, for both selected traits. The six chromosome substitution lines will be used to construct recombinant inbred lines, in which chromosome from the high selection line is recombined with chromosome from the low selection line. The bristle numbers and recombination breakpoints will be determined for each recombinant inbred line, the latter using cytological locations of roo transposable elements, which will differ between high and low parental chromosomes. The numbers, effects, and locations of QTLs affecting the bristle traits will be estimated. The effects of nine cloned major loci which affect bristle development on quantitative variation in bristle number in a natural population will be assessed. Wild type regions from each of these loci from 50 chromosomes of a natural population will be substituted into a common isogenic background, and their contribution to genetic variation in bristle number estimated. Restriction map variation will be determined for the nine loci, and associations between molecular polymorphisms and phenotypic effects will be examined.

长期目标是了解定量性状的变化 单个基因座（QTL）的术语影响它们。 许多人继承了人类 疾病是定量特征。 具体目的是确定 具有重大形态影响的基因座等位基因是否有助于 自然种群的定量差异，并确定 分子和定量遗传之间关联的性质 这些基因座的变化。 要使用的模型系统是 果蝇中的腹部和胸骨刷毛。 胸骨和腹部的发散人工选择线 刚毛的人数将从自然人群开始，并被选为 极限。 主要染色体对选择反应的影响将 被解决。 染色体替代线，其中可行的专业 高选择线的染色体染色体很大 并替换为纯合低选择线背景，将是 为每个主要的染色体制作，用于两个选定的特征。 六个 染色体替代品将用于构建重组近交 线，其中高选择线的染色体与 低选择线的染色体。 鬃毛数量和 将确定每个重组近交的重组断点 线，后者使用ROO转座元素的细胞学位置， 这将在高和低亲代染色体之间有所不同。 数字， 影响和影响刷毛性状的QTL的位置将是 估计的。 九个克隆的主要基因座的影响，影响刷毛的发育对 自然人群中刷毛数的定量变化将是 评估。 来自50个染色体的每个基因座的野生型区域 自然人口将被替换为常见的质基因背景 以及它们对估计猪鬃数量遗传变异的贡献。 限制图的差异将确定为九个基因座，并确定 分子多态性和表型效应之间的关联将是 检查。