Characterisation of a novel bacterial ribonucleoprotein complex analogous to eukaryotic processing (P) bodies in Escherichia coli

类似于大肠杆菌中的真核加工 (P) 体的新型细菌核糖核蛋白复合物的表征

• 批准号: BB/V000284/1
• 负责人: Sivaramesh Wigneshweraraj
• 金额: $ 72.17万
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FV000284%2F1
• 关键词: Characterisation; novel; bacterial; ribonucleoprotein; complex

Bacteria need nutrients to grow. However, in the environment or in the human body, bacterial growth is often compromised by nutrient limitation. Hence, bacteria spend the majority of their time in a starved and thus non-growing state. The processes that allow bacteria to cope with nutrient starvation begin with the synthesis of RNA - the first step in the reaction that switches on genes. Therefore, it is important to understand how the RNA, once synthesized, is managed to allow bacterial cope with nutrient starvation. This will allow us design novel interventional strategies to combat disease causing bacteria. In this project, since nitrogen represents an essential element of most molecules in the bacterial cell, we will use nitrogen starvation as a model nutrient stress to study in detail, how the bacterium Escherichia coli manages RNA. In particular, we will study in detail a novel 'site of RNA storage' in the E. coli, which we discovered to play an important role in how E. coli copes with nitrogen starvation. We posit that this 'site of RNA storage' could be akin to a similar feature, called the P-body, which is often formed in stressed cells found in our bodies and that of other animals. In summary, the results of this project will advance our fundamental knowledge of how the bacterial cell functions and thereby provide us with the much-needed new information and inspiration to control disease causing bacteria.

细菌需要营养才能生长。然而，在环境或人体内，细菌的生长常常受到营养限制的影响。因此，细菌大部分时间都处于饥饿状态，因此无法生长。细菌应对营养饥饿的过程始于RNA的合成——这是开启基因的反应的第一步。因此，了解 RNA 在合成后如何让细菌应对营养饥饿非常重要。这将使我们能够设计新的干预策略来对抗致病细菌。在这个项目中，由于氮是细菌细胞中大多数分子的必需元素，因此我们将使用氮饥饿作为营养应激模型来详细研究大肠杆菌如何管理 RNA。特别是，我们将详细研究大肠杆菌中一个新的“RNA 储存位点”，我们发现它在大肠杆菌应对氮饥饿方面发挥着重要作用。我们假设这个“RNA 储存位点”可能类似于称为 P 体的类似特征，它通常是在我们体内和其他动物体内发现的应激细胞中形成的。总之，该项目的结果将增进我们对细菌细胞如何发挥作用的基础知识，从而为我们提供控制致病细菌急需的新信息和灵感。

项目成果

RNA Management During T7 Infection.

T7 感染期间的 RNA 管理。

• DOI: http://dx.10.1089/phage.2022.0029
• 发表时间: 2022
• 期刊: PHAGE (New Rochelle, N.Y.)
• 作者: Tabib

Global Hfq-mediated RNA interactome of nitrogen starved Escherichia coli uncovers a conserved post-transcriptional regulatory axis required for optimal growth recovery.

氮饥饿大肠杆菌的全局 Hfq 介导的 RNA 相互作用组揭示了最佳生长恢复所需的保守转录后调节轴。

• DOI: http://dx.10.1093/nar/gkad1211
• 发表时间: 2023
• 期刊: Nucleic acids research
• 影响因子: 14.9
• 作者: McQuail J

The association between Hfq and RNase E in long-term nitrogen-starved Escherichia coli.

长期缺氮大肠杆菌中 Hfq 和 RNase E 之间的关联。

• DOI: http://dx.10.1111/mmi.14782
• 发表时间: 2022
• 期刊: Molecular microbiology
• 影响因子: 3.6
• 作者: McQuail J