MECHANISMS OF PHAGE EXCLUSION SYSTEM

噬菌体排除系统的机制

• 批准号: 3280667
• 负责人: IAN J MOLINEUX
• 金额: $ 16.31万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-04-01 至 1995-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3280667
• 关键词: DNA binding protein; DNA gyrase; DNA replication; Escherichia coli; adenosinetriphosphatase; bacteriophage T7; gene expression; genetic mapping; genetic transcription; molecular cloning; mutant; nucleic acid structure; virus genetics; virus infection mechanism; virus protein

The primary objective of this research is to understand the molecular mechanism underlying F-exclusion of T7 and related bacteriophages. This is a model system or host-parasite interactions where the genes involved can be easily manipulated by genetic approaches. Knowledge accrued in this particular exclusion system is being applied to others to determine the generality of the model. Classical and molecular genetic techniques are employed to investigate the pleiotropic physiological dysfunctions associated with F-exclusion. These include perturbations in gene expression, inhibition of DNA replication, and membrane defects. It will be determined if the primary block to phage development is the transcriptionally-mediated entry of phage DNA into the cell. How the phage proteins that are the targets of the exclusion system interact with E. coli DNA gyrase in normal phage development is to be investigated. A genetic selection is described for mutants altered in those host products that are involved in the exclusion system. These mutants should help define the site of interaction of the phage- and plasmid-coded genes and provide information on the primary cellular event that causes the arrest in phage development.

这项研究的主要目的是了解 T7和相关的F-限制基础的分子机制 噬菌体。 这是模型系统或宿主 - 寄生虫相互作用 涉及基因的地方可以通过遗传方法轻松操纵。 在此特定排除系统中累积的知识正在应用于 其他人确定模型的一般性。 经典和分子遗传技术用于 研究与相关的多效性生理功能障碍 f-限制。 这些包括基因表达的扰动，抑制作用 DNA复制和膜缺陷。 将确定是否 噬菌体发展的主要阻滞是转录介导的 将噬菌体DNA进入细胞。 噬菌体蛋白如何 排除系统的靶标与大肠杆菌DNA回旋酶相互作用 应研究正常的噬菌体发展。 描述了在那些宿主中改变的突变体的遗传选择 涉及排除系统的产品。 这些突变体应该 有助于定义噬菌体和质粒编码基因相互作用的位点 并提供有关导致主要蜂窝事件的信息 在噬菌体开发中逮捕。