F FACTOR MEDIATED ABORTIVE INFECTION

F因子介导的流产感染

• 批准号: 3280661
• 负责人: IAN J MOLINEUX
• 金额: $ 8.75万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-04-01 至 1986-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3280661
• 关键词: Escherichia coli; bacteria; bacteriophage T7; female; genetic mapping; genetic regulation; male; mutant; virus genetics; virus infection mechanism

The overall objective of this proposal is to understand the molecular mechanism whereby bacteriophage T7 is restricted upon infection of a male strain of E. coli. This phenomenom is also known as the abortive infection. A new approach is being applied to help solve an old and controversial problem. We have made, via an in vivo recombination experiment, hybrid phages between T7 and the related phage T3. Whereas T7 is restricted by males, T3 is not and these hybrid phages, which are predominately T7 in origin, contain the T3 functions necessary to overcome the abortive infection. We have also recently isolated mutants of T3 which appear to be fully restricted by male strains. Proposed here is a more detailed genetic and physiological characterization of the gybrid phages, designed in particular to separate the two essential functions supplied by T3. These derivatives will be characterized independently on infections of both male and female strains. Similarly the existing T3 mutants, together with others that will be isolated, will be characterized by genetic, physical and physiological criteria. Given these data, in particular knowing precisely which gene products are defective in the T3 mutants, or prevented from functioning (in the case of T7) by the presence of F we can then study the abortive infection itself. For the first time this phenomenom can be investigated by the use of phage mutants and thus the extensive body of knowldege accumulated, on T7 gene products in particular, can be applied to an understanding of the cause of the F factor-mediated abortive infection.

该提议的总体目的是了解分子 噬菌体T7受到雄性感染的机制 大肠杆菌菌株。 这种现象也称为流产 感染。 正在采用一种新方法来帮助解决旧方法 有争议的问题。 我们已经通过体内重组进行了 实验，T7和相关噬菌体T3之间的杂种噬菌体。 而T7 受男性的限制，T3不是，这些混合噬菌体是 原始T7主要包含克服必要的T3功能 流产感染。 我们最近还隔离了T3的突变体 似乎完全受男性菌株的限制。 这里提出的是更多 gybrid噬菌体的详细遗传和生理特征， 特别是为了分开由此提供的两个基本功能 T3。 这些衍生物将以独立于感染的特征 男性和女性菌株。 同样，现有的T3突变体一起 与将会孤立的其他人，将以遗传为特征 物理和生理标准。 给定这些数据，特别是 精确地知道哪些基因产物在T3突变体中有缺陷，或 通过F的存在阻止（在T7的情况下）我们可以 然后研究流产感染本身。 这是第一次 可以通过使用噬菌体突变体来研究现象 广泛的知识范围尤其是在T7基因产品上积累 可以应用于对F因子介导的原因的理解 流产感染。