MOLECULAR MECHANISMS IN DEVELOPMENT

发展中的分子机制

• 批准号: 3278445
• 负责人: THOMAS B. KORNBERG
• 金额: $ 25.86万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-03-01 至 1993-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3278445
• 关键词: DNA binding protein; Drosophilidae; Escherichia coli; alleles; animal population genetics; cell differentiation; developmental genetics; early embryonic stage; embryogenesis; gene expression; genetic library; genetic manipulation; genetic mapping; genetic promoter element; genetic strain; genetic transcription; larva; metamorphosis; molecular cloning; monoclonal antibody; mutant; nonmammalian vertebrate embryology; nucleic acid probes; nucleic acid sequence; radiotracer; regulatory gene; transcription factor

The developmental mutants of Drosophila melanogaster have been a rich and productive source for investigations into basic mechanisms of development. Indeed, the explosive progress in our understanding of the molecular events in early Drosophila embryogenesis already justified the extensive investments in basic genetic and developmental studies that have provided the requisite materials and conceptual foundation. This progress also justifies our optimism that the Drosophila system will continue to provide valuable insights. We have chosen to intensively study one such locus in Drosophila, engrailed. Studies of mutants indicate that the engrailed function is essential during the precellular stages of embryogenesis for the formation of the blastoderm, during the gastrulation period for normal segmentation, and throughout subsequent development for maintenance of the segmental and compartmental subdivisions. The gene has been cloned, the gene structure and transcription unit has been defined, and the engrailed protein has been isolated. This system is now poised for molecular and enzymological studies to establish how the gene is regulated to effects to precise a program of positional and temporal expression, and to determine how the engrailed protein performs its several roles. We will approach these goals by: (1) isolating cis and trans acting mutations that affect the regulation of the engrailed gene; (2) analyzing the cis regulatory elements of the engrailed promoter by DNA transformation, and by isolating and characterizing the enzymes that regulate transcription of the engrailed gene; and (3) studying the activities and behavior of the engrailed protein.

果蝇的发育突变体是一个 富有生产力的来源，用于研究基本机制 发展。 确实，我们的爆炸性进步 了解早期果蝇中的分子事件 胚胎发生已经证明了基本的广泛投资 提供必要的遗传和发育研究 材料和概念基础。 这个进度也证明了 我们对果蝇系统将继续提供的乐观 宝贵的见解。 我们选择在果蝇中深入研究这样一个基因座， 插入。 突变体的研究表明插入的功能 在胚胎发生的胚胎阶段至关重要 在胃胚层的形成期间 正常分割，以及随后的整个开发 维护分段和隔室细分。 这 基因已克隆，基因结构和转录单元具有 已定义，并且已隔离蛋白质。 这 现在，系统有望进行分子和酶学研究 建立如何调节基因以精确程序的影响 位置和时间表的表达，并确定如何 插的蛋白质扮演多个角色。 我们将通过以下方式实现这些目标：（1）隔离顺式和跨 影响调节植物基因的作用突变； （2）分析插入启动子的顺式调节元素 通过DNA转化，并通过隔离和表征 调节植物基因转录的酶； （3） 研究纽约蛋白质的活动和行为。