Gene regulation and function in early embryos

早期胚胎的基因调控和功能

基本信息

批准号：
8840979
负责人：
THOMAS B. KORNBERG
金额：
$ 30.08万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-05-01 至 2018-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8840979
关键词：
Animals Behavior Binding Birth Blastoderm Branchiostoma floridae AmphiEn protein Cell Cycle Cell Cycle Progression Cell Maturation Cell Nucleus Cell-Free System Cells Cessation of life Complement Cytokinesis Detection Development Disease Drosophila genus Elements Embryo Embryonic Development Employee Strikes Fertilization Gene Expression Gene Expression Profile Gene Expression Regulation Genes Genetic Transcription Germ Cells Giant Cells Health Hour Interphase Investigation Lead Life Marriage Methods Molecular Analysis Molecular Genetics Monitor Movement Nature Nuclear Organogenesis Pattern Phenotype Population Process RNA Reading Reagent Role Specimen Staging System Testing Time Transcript Work base cell motility cohort deep sequencing gastrulation gene function genetic analysis improved mutant novel nuclear division programs research study transcription factor transcriptome sequencing

项目摘要

DESCRIPTION (provided by applicant): Cell cycle progression, cell migrations and gene expression that occur at the wrong time or place, or that are inaccurately regulated, can result in abnormal development and can lead to disease states. There are many systems that can be studied to improve our understanding of the cell cycle, cell movements and gene expression. One that has several unique advantages is the early Drosophila embryo when nuclear cycles produce approximately four thousand nuclei within the first two hours after fertilization. These early cycles offer one of the few tractable systems with both high uniformity and an abbreviated and apparently simplified cell cycle. This proposal describes an investigation of these nuclear divisions; findings that are the basis for the proposed experiments already reveal that our understanding of this early, critical stage of development must change radically.

描述（由申请人提供）：在错误的时间或地点发生的细胞周期进程、细胞迁移和基因表达，或者调控不准确，可能会导致发育异常并可能导致疾病状态。有许多系统可以通过研究来提高我们对细胞周期、细胞运动和基因表达的理解。具有几个独特优势的是早期果蝇胚胎，其核周期在受精后的前两个小时内产生大约四千个细胞核。这些早期循环提供了少数易于处理的系统之一，具有高度均匀性和简短且明显简化的细胞周期。该提案描述了对这些核部门的调查；作为拟议实验基础的研究结果已经表明，我们对这一早期关键发展阶段的理解必须从根本上改变。