Unlocking the metabolic potential of the exceptional lignocellulose degrading fungus Parascedosporium putredinis N01

释放特殊木质纤维素降解真菌腐烂副孢子菌 N01 的代谢潜力

• 批准号: BB/W000695/1
• 负责人: Neil Bruce
• 金额: $ 73.26万
• 依托单位: University of York
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FW000695%2F1
• 关键词: Unlocking; metabolic; potential; exceptional; lignocellulose

Lignocellulose provides structure and support to growing plants by enabling strong and durable cell walls. It is one of the most abundant forms of fixed carbon in the biosphere and its breakdown is a critical component of the global carbon cycle. Lignocellulose also represents a vast global resource of sugars and aromatics which can be liberated for the production of biofuels and chemicals. Biorefineries require the use of high yielding, low input biomass crops and/or waste biomass as feedstock; however, the complex phenolic polymers present in lignin make lignocellulose very resistant to enzymatic attack. Current industrial approaches for the breakdown of lignocellulose employ multi-component enzymatic mixtures. While this approach is effective in hydrolysing polysaccharides, it is inefficient and expensive due to the requirement of chemically-intensive pretreatments to disrupt lignin and reduce the crystallinity of cellulose. It is generally recognised that the breakdown of lignin forms the highest barrier to the development of a cost-competitive lignocellulose processing industry, and this barrier will be overcome in part by the discovery of new enzymes for more effective digestion of the feedstock. It is also generally recognised that valorisation of lignin will be a crucial component of integrated biorefineries if they are to be profitable, therefore, it is essential that new strategies for obtaining high value chemicals from lignin are developed. The basis of this project is to gain a detailed understanding of lignocellulose degradation by the fungus Parascedosporium putredinis NO1 and begin to design more efficient industrial approaches to lignin degradation and valorisation. We will carry out a full characterisation of an exciting new ligninase that we have recently discovered that cleaves the major beta-ether structural units in lignin without a requirement of a cofactor. This enzyme not only boosts the breakdown of biomass by commercial cellulase cocktails, it also releases the pharmaceutically important flavonoid tricin from the lignin macromolecule, offering the possibility of producing a valuable product from lignin while reducing the processing costs. We will use 2-dimensional NMR to identify major changes in lignocellulose composition during digestion. We will use comparative proteomics to compare the secretome of P. putredinis NO1 growing on lignin to identify new lignin active enzymes for further study with a particular focus on possible biorefining applications. We will investigate the genes and enzymes expressed, and biochemical processes, in order to identify new enzymes for study. We will produce recombinant forms of these enzymes for characterisation studies. We will use high throughput assay systems to examine the commercial potential of new enzymes for biomass processing. These studies can help us to understand important environmental components of the global carbon cycle, as well as providing us with new understanding and tools to improve the industrial exploitation of lignocellulosic biomass.

木质纤维素通过形成坚固耐用的细胞壁，为生长中的植物提供结构和支持。它是生物圈中最丰富的固定碳形式之一，其分解是全球碳循环的关键组成部分。木质纤维素还代表着全球巨大的糖和芳烃资源，可以将其用于生产生物燃料和化学品。生物精炼厂需要使用高产、低投入的生物质作物和/或废弃生物质作为原料；然而，木质素中存在的复杂酚类聚合物使木质纤维素对酶的攻击具有很强的抵抗力。目前分解木质纤维素的工业方法采用多组分酶混合物。虽然这种方法在水解多糖方面有效，但由于需要化学密集型预处理来破坏木质素并降低纤维素的结晶度，因此效率低且昂贵。人们普遍认为，木质素的分解构成了具有成本竞争力的木质纤维素加工业发展的最高障碍，而这一障碍将通过发现更有效地消化原料的新酶来部分克服。人们还普遍认识到，如果综合生物精炼厂要盈利，木质素的增值将是其关键组成部分，因此，开发从木质素中获取高价值化学品的新策略至关重要。该项目的基础是详细了解真菌腐烂副孢菌 NO1 的木质纤维素降解作用，并开始设计更有效的木质素降解和增值工业方法。我们将对一种令人兴奋的新型木质素酶进行全面表征，我们最近发现这种酶可以在不需要辅因子的情况下裂解木质素中的主要β-醚结构单元。这种酶不仅可以促进商业纤维素酶混合物对生物质的分解，还可以从木质素大分子中释放出药学上重要的黄酮类三辛酸，从而提供了从木质素生产有价值的产品的可能性，同时降低了加工成本。我们将使用二维核磁共振来识别消化过程中木质纤维素成分的主要变化。我们将使用比较蛋白质组学来比较在木质素上生长的 P. putredinis NO1 的分泌组，以确定新的木质素活性酶，以供进一步研究，特别关注可能的生物精炼应用。我们将研究表达的基因和酶以及生化过程，以便识别新的酶进行研究。我们将生产这些酶的重组形式用于表征研究。我们将使用高通量测定系统来检查用于生物质加工的新酶的商业潜力。这些研究可以帮助我们了解全球碳循环的重要环境组成部分，并为我们提供新的认识和工具来改善木质纤维素生物质的工业利用。

项目成果

Whole genome structural predictions reveal hidden diversity in putative oxidative enzymes of the lignocellulose-degrading ascomycete Parascedosporium putredinis NO1.

• DOI: 10.1128/spectrum.01035-23
• 发表时间: 2023-12-12
• 期刊: MICROBIOLOGY SPECTRUM
• 影响因子: 3.7
• 作者: Scott, Conor J. R.;Leadbeater, Daniel R.;Oates, Nicola C.;James, Sally R.;Newling, Katherine;Li, Yi;Mcgregor, Nicholas G. S.;Bird, Susannah;Bruce, Neil C.;Cha, Chang-Jun
• 通讯作者: Cha, Chang-Jun

A bioinformatic workflow for in silico secretome prediction with the lignocellulose degrading ascomycete fungus Parascedosporium putredinis NO1.

利用木质纤维素降解子囊菌真菌 Parascedosporium putredinis NO1 进行计算机分泌蛋白组预测的生物信息学工作流程。

• DOI: 10.1111/mmi.15144
• 发表时间: 2023
• 期刊: Molecular microbiology
• 影响因子: 3.6
• 作者: Scott CJR