Automated high throughput high-resolution imaging

自动化高通量高分辨率成像

• 批准号: BB/V019481/1
• 负责人: Corinne Houart
• 金额: $ 47.36万
• 依托单位: King's College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FV019481%2F1
• 关键词: Automated; throughput; resolution; imaging

Solving essential questions of biology requires the acquisition of large amount of data and excellent analytical approaches. For research addressing biological problems using tissues and animals, the pace of progress is mostly hampered by the lack of technologies allowing to acquire and analyse large amount of images at high resolution in a short period of time. Such technology has recently been built, in the form of a computer-controlled robotic device coupled to a confocal microscope. This high throughput equipment, called VAST, can automatically load, orient, image and redistribute hundreds of live embryos into multiwell plates. Imaging is done at subcellular resolution in record time, while keeping track of all the data to the redistributed animals for further studies. The computer not only acquire the data but also analyse them according to parameters defined by the specific research question investigated. Such equipment is to yet available in the UK, except for Edinburgh. We therefore propose to acquire such equipment in London, for the benefit of a large set pf scientific programmes supported by the BBSRC. Moreover, we plan to open the equipment to a broader scientific community, in and around the capital (eg. Brighton, Cambridge, Bath, Bristol).

解决生物学的基本问题需要获取大量数据和出色的分析方法。对于使用组织和动物解决生物学问题的研究来说，进展速度主要受到缺乏能够在短时间内获取和分析大量高分辨率图像的技术的阻碍。这种技术最近以计算机控制的机器人设备与共焦显微镜耦合的形式出现。这种高通量设备称为 VAST，可以自动将数百个活胚胎装载、定向、成像并将其重新分配到多孔板中。成像以亚细胞分辨率在创纪录的时间内完成，同时跟踪重新分配的动物的所有数据以供进一步研究。计算机不仅获取数据，还根据所调查的具体研究问题定义的参数对数据进行分析。除爱丁堡外，英国尚未提供此类设备。因此，我们建议在伦敦购买此类设备，以便 BBSRC 支持的大量科学项目受益。此外，我们计划向首都及其周边地区（例如布莱顿、剑桥、巴斯、布里斯托尔）更广泛的科学界开放这些设备。

项目成果

Loss of slc39a14 causes simultaneous manganese hypersensitivity and deficiency in zebrafish.

• DOI: 10.1242/dmm.044594
• 发表时间: 2022-06-01
• 期刊: Disease models & mechanisms
• 影响因子: 4.3

Prematurely terminated intron-retaining mRNAs invade axons in SFPQ null-driven neurodegeneration and are a hallmark of ALS.

• DOI: 10.1038/s41467-022-34331-4
• 发表时间: 2022-11-22
• 期刊: NATURE COMMUNICATIONS
• 影响因子: 16.6
• 作者: Taylor, Richard;Hamid, Fursham;Fielding, Triona;Gordon, Patricia M.;Maloney, Megan;Makeyev, Eugene, V;Houart, Corinne
• 通讯作者: Houart, Corinne