SUMOcode: deciphering how SUMOylation enables plants to adapt to their environment

SUMOcode：解读 SUMOylation 如何使植物适应环境

• 批准号: BB/V003534/1
• 负责人: Ari Sadanandom
• 金额: $ 464.75万
• 依托单位: Durham University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FV003534%2F1
• 关键词: SUMOcode; deciphering; how; SUMOylation; enables

At a basic level the rules that govern life is defined by an organism's genetic code (like the text of a book) and the signals it receives from the environment (like the interpretation of the reader of the text). The combination of these two (the genetic code and the signals) makes an organism behave and develop the way it does. At a molecular level there are several critical systems that control the way an organism responds to its environment. These can be (1) long-term responses through the silencing of the genetic code (like removing parts of the text in the book so it can't be interpreted) or (2) more rapid responses through modifications of the system (like annotation to parts of the text of a book which changes the way it is interpreted). Understanding these responses is essential to our understanding of how an organism functions and how an organism changes based on their environment. Here we are focusing on the second type of response, which at a molecular level are initiated by "post-translational modifications (PTMs)" (this a way of changing the function of the existing machinery in a cell). PTMs act at the core of every biological system. Taking signals from outside the cell and "coding" molecular interactions to change the way cells function. This is critical in every biological process. There are several types of PTMs, but one of the most important but whose code is not defined is SUMOylation. Here we aim to take a holistic approach to understanding the SUMO code.In this programme we will develop a SUMO machinery cell atlas (a resource that will characterize each part of the machinery), how, in which cells and when it works, so that a map of the key events that trigger a SUMOylation response to environmental cues can be revealed. We will use the model plant, Arabidopsis, arguably the best non-human, multicellular organism for this scale of interrogation. It has a plethora of tools and resources that will allow us to dissect the SUMO code in detail and across different cell types, different stages of development and across different response times. This mapping of SUMOylation will reveal the 'hubs' that the SUMO machinery targets to cause a cellular response, revealing how the pathway functions and how it can be manipulated to combat environmental challenges or disease. SUMOylation has already been shown by our group and others that it is important for the way a cell responds to environmental stresses. For example, plants adapt to changes in their environment (heat, water availability, salt, etc) by modifying their growth and development (to enhance their ability to survive and flourish), through PTMs like SUMOylation. Therefore, a key output of this programme will be a set of tools that will translate the SUMO language across the plant kingdom and provide insights into animal and human health and disease. Our ultimate goal is to 'enable' researchers from a range of disciplines, plant breeders, chemical companies and beyond to edit the SUMO code discovered here to improve crop resilience, future proofing them against ongoing climate instability and change, and to catalyse new insights across plants and animals into the rules that govern an organisms behaviour and responses to the environment that surrounds them.

在基本层面上，控制生命的规则是由生物体的遗传密码（如书籍的文本）及其从环境中收到的信号（例如文本读者的解释）所定义的。这两个（遗传密码和信号）的结合使有机体行为并发展其方式。在分子层，有几个关键系统可以控制生物体对其环境的反应方式。这些可以是（1）通过沉默遗传密码（例如删除书中文本中的一部分，因此无法解释）或（2）（2）通过修改系统（例如对本书的一部分的注释，以改变其解释方式））。了解这些反应对于我们对生物体如何运作以及生物体如何根据其环境而变化至关重要。在这里，我们关注第二种响应，在分子级别上，它是通过“翻译后修饰（PTM）”（这是改变单元格中现有机械功能的一种方式）启动的。 PTMS在每个生物系统的核心中。从细胞外部获取信号，并“编码”分子相互作用来改变细胞功能的方式。这在每个生物过程中都是至关重要的。 PTM有几种类型，但最重要的是，但未定义的代码是Sumoylation。在这里，我们旨在采用一种整体方法来理解Sumo代码。在此程序中，我们将开发出一个相扑机械单元格（一种将表征机械的每个部分），如何在哪些单元格和何时工作的情况下进行表征，以便可以揭示触发Sumoylation响应的关键事件图。我们将使用模型植物，拟南芥，可以说是这种审讯规模的最好的非人类多细胞生物。它具有大量的工具和资源，可以使我们能够详细介绍相扑代码，以及不同的单元格类型，开发的不同阶段以及跨不同响应时间。 Sumoylation的这种映射将揭示出Sumo机械靶向引起细胞反应的“枢纽”，从而揭示了该途径的功能以及如何操纵以应对环境挑战或疾病的操纵。我们的小组和其他人已经证明了Sumoylation对细胞对环境压力的反应方式很重要。例如，植物通过修改其生长和发育（以增强其生长和发展的能力），通过Sumoylation（例如Sumoylation）来适应其环境变化（热量，水量，盐等）。因此，该计划的关键输出将是一组工具，可以将Sumo语言转换为整个植物王国，并提供对动物和人类健康和疾病的见解。我们的最终目标是从一系列学科，植物育种者，化学公司及其他地区“启用”研究人员，以编辑此处发现的Sumo Code，以提高农作物的弹性，将来证明他们免受持续的气候不稳定和变化的态度，并将各种动植物的新见解促进了对环境的行为和对环境的影响的规则。

项目成果

Turning up the volume: How root branching adaptive responses aid water foraging.

• DOI: 10.1016/j.pbi.2023.102405
• 发表时间: 2023-06
• 期刊: Current opinion in plant biology
• 影响因子: 9.5
• 作者: P. Mehra;Rebecca Fairburn;N. Leftley;Jason Banda;M. Bennett

Non-invasive hydrodynamic imaging in plant roots at cellular resolution.

• DOI: 10.1038/s41467-021-24913-z
• 发表时间: 2021-08-03
• 期刊: Nature communications
• 影响因子: 16.6
• 作者: Pascut FC;Couvreur V;Dietrich D;Leftley N;Reyt G;Boursiac Y;Calvo-Polanco M;Casimiro I;Maurel C;Salt DE;Draye X;Wells DM;Bennett MJ;Webb KF
• 通讯作者: Webb KF

The conjugation of SUMO to the transcription factor MYC2 functions in blue light-mediated seedling development in Arabidopsis.

• DOI: 10.1093/plcell/koac142
• 发表时间: 2022-07-30
• 期刊: The Plant cell