Charting the protein modifications systems that underpin submergence tolerance in rice

绘制支撑水稻耐淹性的蛋白质修饰系统

• 批准号: BB/R002754/1
• 负责人: Ari Sadanandom
• 金额: $ 48.96万
• 依托单位: Durham University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FR002754%2F1
• 关键词: Charting; protein; modifications; systems; underpin

Climate change models predict that large areas of Northern Europe and Asia is likely to experience flooding in the next 50 years. Currently flooding affects rice cultivation across 20 million hectares in Asia. To stabilize crop yields it is imperative to increase the capacity of cereal and vegetable crops to endure partial to complete submergence. This has been feasible for rice, due to the identification of the SUBMERGENCE 1A gene (SUB1A, (encoding a group VII ETHYLENE RESPONSE FACTOR [ERFVII] transcription factor) providing tolerance to complete submergence for over 7 days. The molecular characterization of SUB1A has begun to provide new insights into molecular, physiological and developmental processes that underlie an effective submergence survival strategy in plants. While SUB1A is important to ensure rice productivity in vast flood prone areas of the world, recent increases in frequency and magnitude of submergence due to extreme flooding events across Asia has negatively impacted SUB1A efficacy. New knowledge and resources are urgently needed that can boost SUB1A efficacy in mediating flooding tolerance. Recent evidence indicates that a difference in levels of flooding tolerance of SUB1A introgressed lines may be due to either transcriptional or post-transcriptional mechanisms that affect SUB1A activity. Our preliminary evidence suggests that the stability of the SUB1A protein is likely to be critical in mediated prolonged submergence tolerance in rice.The project partners have discovered that SUB1A protein stability (and therefore activity) is affected by two major post-translational mechanisms, ubiquitylation and SUMOylation. By exploiting this new knowledge we aim to pave the way to develop new rice varieties in which the SUB1A protein is more stable and active during prolonged flooding, generates enhanced submergence tolerance and therefore greater stability of yield. This proposal also aims to exploit the project partners unique insight and world leads into post-translational mechanisms (PTMs), including N-end rule based Ubiquitylation and SUMOylation, to identify new molecular regulators that control submergence response in plants. This could lead the development of a new generation of flooding tolerant crop varieties.Overarching hypotheses of the proposal: 1. Manipulating protein modifications that govern SUB1A stability can lead to extended flooding tolerance in rice. 2. Protein modification systems can be exploited to identify new regulators of submergence tolerance in rice.These 2 hypotheses will be tested by 3 main objectives within the 3 year project.1. To investigate the interaction between the molecular and environmental factors that affect SUB1A stability and posttranslational state. 2. To test for submergence tolerance and (time permitting) cross-tolerance to multiple abiotic stresses in transgenic rice plants that manipulate the regulatory components of SUB1A stability. 3. Discover novel regulators of flooding tolerance by exploiting preliminary data that indicates the presence of new targets that affects plant survival and undergo both N-end rule mediated Ubiquitylation and SUMOylation during submergence induced hypoxia.Timeliness: Our preliminary observations demonstrating that posttranslational modifications are essential for SUB1A function provide a highly novel molecular explanation and conduit to enhanced flooding tolerance in rice. Characterization of the role of the PTMs in controlling SUB1A and definition of new regulators of submergence with solved molecular mechanisms will offer novel approaches for delivery of plants with robust flooding and wider abiotic stress tolerance.

气候变化模型预测，在未来50年内，北欧和亚洲的大片地区可能会遭受洪灾。目前，洪水影响亚洲2000万公顷的水稻种植。为了稳定农作物的产量，必须增加谷物和蔬菜作物的能力以忍受部分浸没。由于鉴定了1A基因（SUB1A（编码VII组的乙烯反应因子[ERFVII]转录因子），这对大米来说是可行的，从而提供了7天的完全浸没的耐受性。SUB1A的分子表征已开始提供新的洞察力，而在生存中，sib1a的分子表征已开始，以实现生存和生存为生存。 Sub1a对于确保巨大的洪水泛滥地区的水稻生产力很重要，由于亚洲的极端洪水事件而导致的淹没频率和大小对SUB1A的效力产生了负面影响，因此迫切需要新的知识和资源，这可以提高SUB1A的效力，从而在造成洪水范围的范围内降低了洪水量的差异。我们的初步证据的转录后机制表明，SUB1A蛋白的稳定性在水稻中介导的长时间浸没耐受性可能至关重要。通过利用这一新知识，我们旨在为开发新的水稻品种铺平道路，在长期洪水期间，SUB1A蛋白更稳定和活跃，从而产生增强的淹没耐受性，从而产生更大的产量稳定性。该提案还旨在利用项目合作伙伴独特的见解，而世界则导致翻译后机制（PTMS），包括基于N端规则的泛素化和Sumoylation，以识别控制植物中累积反应的新分子调节剂。这可能会导致新一代洪水耐受的作物品种的发展。该提案的假设：1。操纵控制SUB1A稳定性的蛋白质修饰可以导致大米的泛滥耐受性。 2。可以利用蛋白质修饰系统来确定大米中浸没耐受性的新调节剂。这些假设将在3年项目中通过3个主要目标测试。1。研究影响亚1a稳定性和翻译后状态的分子和环境因素之间的相互作用。 2。用于测试转基因水稻植物中多种非生物胁迫的浸没耐受性和（时间允许）操纵SUB1A稳定性的调节成分的多种非生物胁迫。 3。通过利用初步数据来发现洪水耐受性的新型调节因子，该数据表明存在影响植物生存的新目标，并经历了n端规则介导的泛素化和降级引起的缺氧期间的泛素化和sumoylation。大米的耐受性。 PTM在控制SUB1A中的作用以及使用已解决的分子机制的新调节剂的定义的表征将提供新颖的方法，以供植物散布具有强大的洪水和更大的非生物胁迫耐受性。

项目成果

An Insight into the Factors Influencing Specificity of the SUMO System in Plants.

• DOI: 10.3390/plants9121788
• 发表时间: 2020-12-17
• 期刊: Plants (Basel, Switzerland)
• 作者: Srivastava M;Sadanandom A
• 通讯作者: Sadanandom A

BTB-BACK Domain Protein POB1 Suppresses Immune Cell Death by Targeting Ubiquitin E3 ligase PUB17 for Degradation.

• DOI: 10.1371/journal.pgen.1006540
• 发表时间: 2017-01
• 期刊: PLoS genetics
• 影响因子: 4.5
• 作者: Orosa B;He Q;Mesmar J;Gilroy EM;McLellan H;Yang C;Craig A;Bailey M;Zhang C;Moore JD;Boevink PC;Tian Z;Birch PR;Sadanandom A
• 通讯作者: Sadanandom A