PHOSPHOLIPID VESICLES--MODEL FOR BIOLOGICAL MEMBRANES

磷脂囊泡——生物膜模型

• 批准号: 3269106
• 负责人: CHING-HSIEN HUANG
• 金额: $ 15.02万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-05-01 至 1992-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3269106
• 关键词: Raman spectrometry; X ray crystallography; acyl group; analytical ultracentrifugation; calorimetry; chemical structure; chemical structure function; cis trans isomerization; densitometry; diacylglycerols; gel filtration chromatography; high performance liquid chromatography; lysolecithins; membrane fusion; membrane lipids; membrane model; membrane permeability; membrane reconstitution /synthesis; membrane structure; methyl group; molecular asymmetry; nuclear magnetic resonance spectroscopy; phase change; phosphatidylcholines; protein kinase C; thermodynamics; water solution

The long-term objective of this research project is an in-depth investigation of the kinetic, functional, structural, and thermodynamic properties of various chemically well-defined phospholipids in model membrane systems. Specifically, our experiments are designed along the following lines: (1) We have recently applied high-sensitivity differential scanning calorimetry, laser Raman spectroscopy, 31p- and 14N-NMR spectroscopy, ultracentrifugation, X-ray diffraction, together with generl bochemical techniques, to invetigate asymmetric mixed-chain phosphatidylcholine (PC) dispersions. We shall continue our investigation on the phase begavior, structure an dynamics of various PCs in which the sn-2 acyle chain is only half as long as the sn-1 acyl chain. These PCs are most likely to form mixed interdigitated bilayers at T(Tm. (2) We aim to study the kinetics of the metastable gel stable subgel transition. (3) We shall examine the polymorphic phase begavior, the structure, and dynamics of lysophosphatidy lethanolamine and its N-methyl derivatives. (4) We shall investigate the effects of mixed-chain phospholipide and diachylglycerols on the enzyme activity and kinetics of protein kinase C. (5) We shall develop a theoretical model to describe the phase tansition behavior of interdigitated bilayer. Information gained from this proposal will add a new dimension to our current knowledge about the structure, dynamics, and functions of membrane lipids.

该研究项目的长期目标是深入 研究动力学，功能，结构和 各种化学明确的热力学特性 模型膜系统中的磷脂。 具体来说，我们的 实验沿以下几行设计：（1）我们有 最近应用高敏性差异扫描 量热法，激光拉曼光谱，31p-和14n-nmr 光谱，超速离心，X射线衍射，一起 使用Generl Bochemical Techniques，开启不对称 混合链磷脂酰胆碱（PC）分散剂。 我们将 继续我们对阶段的调查，结构 SN-2 Acyle链仅一半的各种PC的动力学 只要SN-1酰基链。 这些PC最有可能形成 在T（TM。（2））的混合互插性双层均旨在研究 亚稳态凝胶稳定子凝胶过渡的动力学。 （3）我们 应检查多态相位的乞avior，结构和 溶物磷脂的动力学及其N-甲基的动力学 衍生物。 （4）我们将研究混合链的影响 酶活性上的磷脂和二乙酰甘油 蛋白激酶C的动力学C（5）我们将发展理论 描述互换的相位键合行为的模型 双层。 从该提案中获得的信息将增加一个新的 我们当前对结构的知识的维度， 膜脂质的动力和功能。