PHOSPHOLIPID VESICLES--MODEL FOR BIOLOGICAL MEMBRANES

磷脂囊泡——生物膜模型

• 批准号: 2734331
• 负责人: CHING-HSIEN HUANG
• 金额: $ 22.71万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-05-01 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2734331
• 关键词: Raman spectrometry; X ray crystallography; calorimetry; chemical chain length; chemical synthesis; fatty acylation; gel filtration chromatography; lipid bilayer membrane; lipid structure; membrane lipids; membrane structure; nuclear magnetic resonance spectroscopy; phosphatidylcholines; phosphatidylethanolamines; phosphoglycerides; phospholipids; stereochemistry; thermodynamics; vesicle /vacuole

DESCRIPTION: Biological membranes contain a substantial amount of PC and PE in which the sn-2 acyl chains are unsaturated hydrocarbons. There is increasing evidence that skeletal muscle phospholipids with specific unsaturated sn-2 acyl chains may be of great significance in modulating the insulin receptor activity (Borkman et al. (1993), NEJM 328, 238; Liu et al. (1994) Biochem. J. 299,831). Also, an optimal function of the brain or retina depends critically on the presence of a highly unsaturated species of phospholipids (Salen & Niebylsk (1995), Mol. Memb. Biol. 12, 131). At present, knowledge of the mechanism by which specific unsaturated phospholipids elicit their essential roles in cell membranes is unknown. It is thus relevant to investigate the structure/property of unsaturated phospholipids, since results obtained may build a mechanistic foundation from which clinically observed abnormalities resulting from the deficiency of unsaturated membrane lipids may be assessed. In the past, this group has gained considerable experience in synthesizing and characterizing the structure/property of saturated mixed-chain PC and PE. Now, the long term objective is to extend the earlier work by directing the efforts towards a new direction, aiming at the elucidation of the structure/property of unsaturated PE and PC in the bilayer. Specifically, PC and PE with distinct chain length, degree of unsaturation, and double bond positions, which resemble strictly the naturally occurring phospholipids, will be synthesized, followed by thermodynamic and structural studies using principally the methods of differential scanning calorimetry and molecular mechanics calculations. In addition, the lipid chain interdigitation of PC induced by alcohols will be examined systematically. Finally, the thermotropic phase behavior of mixed-chain phosphatidylglycerols will be compared with that of PC. Dr. Huang believes that the results of the proposed studies will make genuine advances in the understanding of membrane lipids; in addition, they may lead to many informative new experiments in the future.

描述：生物膜包含大量PC和PE 其中SN-2酰基链是不饱和烃。 有 越来越多的证据表明骨骼肌磷脂具有特定的 不饱和的SN-2酰基链在调制该链中可能具有重要意义 胰岛素受体活性（Borkman等人（1993），NEJM 328，238; Liu等。 （1994）生物化学。 J. 299,831）。 另外，大脑的最佳功能或 视网膜严重取决于高度不饱和物种的存在 磷脂（Salen＆Niebylsk（1995），Mol。Memb。Biol。12，131）。 在 现在，了解特定不饱和的机制 磷脂在细胞膜中引起其重要作用是未知的。 它 因此与研究不饱和的结构/特性有关 磷脂，由于获得的结果可能会建立机械基础 缺乏症引起的临床观察到异常 可以评估不饱和膜脂质的脂质。 过去，这个小组有 在综合和表征综合和表征方面获得了丰富的经验 饱和混合链PC和PE的结构/特性。 现在，长期 目的是通过将努力指导到一个 新方向，旨在阐明 双层中的不饱和PE和PC。 具体而言，PC和PE具有独特的 链长，不饱和度和双键位置，这些位置 严格类似于天然存在的磷脂，将是 合成，然后使用热力学和结构研究 主要是差异扫描量热法和分子的方法 力学计算。 另外，PC的脂质链插头 醇诱导的将被系统地检查。 最后， 混合链磷脂酰甘油的热相性行为将是 与PC相比。 黄博士认为 拟议的研究将在理解膜方面取得真正的进步 脂质；此外，它们可能会导致许多内容丰富的新实验 未来。