PHOSPHOLIPID VESICLES--MODEL FOR BIOLOGICAL MEMBRANES

磷脂囊泡——生物膜模型

• 批准号: 3269109
• 负责人: CHING-HSIEN HUANG
• 金额: $ 10.08万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-05-01 至 1987-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3269109
• 关键词: Raman spectrometry; analytical ultracentrifugation; calorimetry; chemical structure function; cholesterol; cis trans isomerization; densitometry; gangliosides; gel filtration chromatography; lysolecithins; membrane fusion; membrane lipids; membrane model; membrane permeability; membrane reconstitution /synthesis; membrane structure; molecular asymmetry; nuclear magnetic resonance spectroscopy; phase change; phosphatidylcholines; thermodynamics; water solution

The long-term objective of this research project is the investigation of the lipid-lipid interactions in phospholipid bilayers. Specifically, we aim to (1) investigate the gel liquid less than and greater than liquid crystalline phase transition of a wide range of saturated liked-chain phosphatidylcholines (PCs) in aqueous dispersions. The study will utilize highly purified (typically 99 mol %) PCS and involve the thermodynamic (delta S and delta V) and structural (gauche/trans ratio, bilayer or micelle organization) measurements; (2) study the possible coupling between the outer and the inner monolayer of the mixed-chain PC bilayers. The mixed-chain PCs to be employed for this investigation are highly asymmetrical, namely, the number of carbon atoms in sn-2 chain is smaller than that in sn-1 by four or more such as C(18):C(14)-PC and C(18):C(12)-PC; (3) investigate saturated PC vesicle-vesicle aggregation/fusion at tempeature below the phase transition temperature (Tm); (4) continue our investigation of the role of cholesterol in bilayers; particularly, the interaction of cholesterol with lyso PC and various gangliosides. Principal techniques to be used are high-sensitivity differential scanning calorimetry, differential densimetry, analytical ultracentrifuge, NMR and Raman spectroscopy. Information gained from this proposal will add new dimension of our current knowledge about lipid-lipid interaction in biological membranes.

该研究项目的长期目标是调查 磷脂双层中的脂质脂质相互作用。 具体来说，我们 目的是（1）研究凝胶液体小于且大于液体的凝胶液体 各种饱和链的结晶相变 水分散体中的磷脂酰胆碱（PC）。 该研究将利用 高度纯化（通常为99 mol％）PC，涉及热力学 （delta s和delta v）和结构（gauche/trans比，双层或 胶束组织）测量； （2）研究之间可能的耦合 混合链PC双层的外部和内部单层。 这 用于此研究的混合链PC高度高 不对称，即，SN-2链中的碳原子数量较小 在SN-1中比四个或更多的c（18）：c（14）-pc和 C（18）：C（12）-pc; （3）研究饱和的PC囊泡 在相变温度以下的温度下的聚集/融合 （TM）; （4）继续我们调查胆固醇在 双层；特别是，胆固醇与Lyso PC和 各种神经节苷脂。 要使用的主要技术是高灵敏的 差异扫描量热法，差异度计，分析 超速离心，NMR和拉曼光谱。 从中获得的信息 提案将增加我们当前有关脂质脂质的知识的新维度 生物膜中的相互作用。