HEMORRHAGIC AND NECROTIC TOXINS IN SNAKE VENOMS

蛇毒中的出血性和坏死性毒素

• 批准号: 3563669
• 负责人: ANTHONY T TU
• 金额: $ 12.56万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-06-01 至 1992-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3563669
• 关键词: Raman spectrometry; adenosinetriphosphatase; antidotes; atomic absorption spectrometry; chemical structure function; collagenase; conformation; dialysis; drug metabolism; electrofocusing; electron microscopy; erythrocyte membrane; gel electrophoresis; gel filtration chromatography; hemorrhage; high voltage electrophoresis; histology; hyaluronidase; ion exchange chromatography; kidney necrosis; membrane model; metals; microscopy; muscle necrosis; necrosis; peptidases; platelet aggregation; proteolysis; reptile poison; sarcoplasmic reticulum; toxin; zinc

Snakebite is a common occurrence in many parts of the world, paticularly in tropical ares. The venoms of certan snakes, especially the Crotalid family, which includes rattlesnakes, cooperheads, and water mocassins in the United States, causes extensive tissue damage, which in many cases leads to dysfunction or complete loss of extremity. Our objective is to determine the chemical and pharmavological properties of the toxins responsible for hemorrhage and necrosis and the mechanisms of tissue damage on envenomation. Different hemorrhagic toxins and myotoxins will be isolated from snake venoms and their effects on artificial membranes, platelet aggregation, isolated endothelial cells, and sarcoplasmic reticulum will be investigated. In order to establish structure- function relationships of myotoxin a, the toxin wll be modified using several methods and its effect on myonecrotic activity and SR calcium-ATPase activity will be investigated. For the long-term goal, we hope that our research will contribute to the long-range objective of better snakebite treatment, especially n the prevention of tissue damage. Also, by developing the experimental model for this research, we predict their applicability to the study of myodegenerating afflictions and hemorrhagic disorders of man.

蛇咬伤在世界许多地方都很常见， 特别是在热带地区。 某些蛇的毒液， 尤其是响尾蛇科，其中包括响尾蛇， 美国的库珀头和水莫卡辛，导致 广泛的组织损伤，在许多情况下会导致功能障碍 或肢体完全丧失。 我们的目标是确定化学和药理学 导致出血和坏死的毒素的特性 以及毒液造成组织损伤的机制。 不同的 从蛇体内分离出出血毒素和肌肉毒素 毒液及其对人工膜、血小板的影响 聚集、分离的内皮细胞和肌浆 网状结构将被调查。 为了建立结构- 肌毒素a的功能关系，毒素将被修饰 使用几种方法及其对肌坏死活动的影响 将研究 SR 钙-ATP 酶活性。 为了长远目标，我们希望我们的研究能够做出贡献 为了更好地治疗蛇咬伤的长期目标， 尤其是预防组织损伤。 此外，通过开发本研究的实验模型，我们 预测它们在肌变性研究中的适用性 人类的痛苦和出血性疾病。

项目成果

Isolation and characterization of fibrinogenase from western diamondback rattlesnake venom and its comparison to the thrombin-like enzyme, crotalase.

西方小菜斑响尾蛇毒液中纤维蛋白原酶的分离和表征及其与类凝血酶克罗塔酶的比较。

• DOI: 10.1016/0305-0491(83)90377-2
• 发表时间: 1983
• 期刊: Comparative biochemistry and physiology. B, Comparative biochemistry
• 作者: Nikai,T;Kito,R;Mori,N;Sugihara,H;Tu,AT
• 通讯作者: Tu,AT

Hemorrhage induced by snake venoms.

蛇毒引起的出血。

• 发表时间: 1982
• 期刊: Taiwan yi xue hui za zhi. Journal of the Formosan Medical Association
• 作者: Tu,AT

Interaction of myotoxin a with the Ca2+-ATPase of skeletal muscle sarcoplasmic reticulum.

肌毒素 a 与骨骼肌肌浆网 Ca2-ATP 酶的相互作用。

• DOI: 10.1016/0003-9861(86)90452-2
• 发表时间: 1986
• 期刊: Archives of biochemistry and biophysics
• 影响因子: 3.9
• 作者: Volpe,P;Damiani,E;Maurer,A;Tu,AT
• 通讯作者: Tu,AT

A calorimetric examination of the effect of myotoxin a on the thermotropic phase behavior of model lipid membranes.

肌毒素 a 对模型脂质膜热致相行为影响的量热检查。

• DOI: 10.1016/0009-3084(87)90042-9
• 发表时间: 1987
• 期刊: Chemistry and physics of lipids
• 影响因子: 3.4
• 作者: Liddle,WK;Middaugh,CR;Tu,AT
• 通讯作者: Tu,AT

Purification and characterization of a fibrinogenase from the venom of Western Diamondback rattlesnake (Crotalus atrox).

西方响尾蛇 (Crotalus atrox) 毒液中纤维蛋白原酶的纯化和表征。

• DOI: 10.1016/0167-4838(83)90101-2
• 发表时间: 1983
• 期刊: Biochimica et biophysica acta
• 作者: Sapru,ZZ;Tu,AT;Bailey,GS
• 通讯作者: Bailey,GS