Development of next generation vaccine technology inducing rapid and strong immunity through targeted delivery of antigens to chicken immune cells

开发下一代疫苗技术，通过将抗原定向递送至鸡免疫细胞，诱导快速而强大的免疫力

基本信息

批准号：
BB/T013087/1
负责人：
Munir Iqbal
金额：
$ 60.28万
依托单位：
The Pirbright Institute
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2021
资助国家：
英国
起止时间：
2021 至无数据
项目状态：
未结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FT013087%2F1
关键词：
Development next generation vaccine technology

项目摘要

Safe and disease-free poultry production systems are crucial for maintaining food supplies to feed the increasing human population and to improve the livelihoods of farming communities. However, the biggest threat in the sustainable growth of farmed animal production is the emergence, re-emergence and continual prevalence of a multitude of infectious animal pathogens, such as avian influenza. Primarily, the control against these pathogens is achieved through vaccination. However, the effectiveness of most of the current vaccines is suboptimal, where they may only reduce manifestation of clinical disease and mortality, but infected animals continue to shed viruses resulting in a continual chain of infections to susceptible naïve and vaccinated animals. For example, despite the large-scale deployment of multiple doses of influenza vaccine to an individual bird, manifestation of disease continues in the form of endemic prevalence and farmers continue to bear debilitating losses with up to 100 percent flock mortality or loss of egg production along with the threat of zoonotic infections. Therefore, improvement of the current vaccines are required that produce stronger immunity and full protection against disease, together with a reduction in shedding of infectious virus from infected animals, preventing the endemic prevalence of these viruses in farmed animals. In this proposal, we plan to develop robust and effective vaccines that elicit strong and durable humoral and cellular immune responses against viral pathogens which cause severe economic losses in poultry industries. As a proof-of-principle, we have demonstrated that targeted delivery of antigens to chicken immune cells potentiate the antigen-specific immune responses in vaccinated chickens compared to the untargeted counterpart or the conventional killed virus vaccines. The targeted vaccines demonstrated a significantly faster and a significantly higher immune response. These studies therefore provided strong data to take this new vaccine technology from laboratory to the farm. To achieve this goal, we have established partnership with a commercial poultry vaccine producer "MSD Animal Health". Through this collaboration, we will investigate how our developed targeted antigen delivery vaccine (TADV) formulation can further be optimised to i) induce even more rapid immunity and provide more potent and broader immune responses against antigenic AIV variants infecting poultry in different geographic regions; ii) enhance the production and delivery methods in chickens using our well-established insect cell cultures and recombinant viral vector (herpesvirus of turkey) system. The result obtained from this proposed research would provide a novel next generation improved AIV vaccine and a platform for improvement of vaccine technology against other important livestock and human pathogens. Availability of these novel highly protective and cost-effective disease control tools and strategies should minimise the impact of infectious diseases on farm animals, and offer substantial indirect economic, public health, environmental and social benefits to the UK and rest of the world.

安全和无疾病的家禽生产系统对于维持粮食供应至关重要通过当前疫苗的疫苗的持续流行率是次优的ISEase和死亡率，但是感染的动物继续散发出病毒，导致感染的持续链，导致感染链接到敏感性的动物 - 将多种剂量的流感疫苗部署到一个流行的单独的鸟类RM，农民与100％至100％的鞭locge菌一起产生了currense的电流。，我们计划在农场中的特有疾病的流行率，我们计划开发出强大而有效的疫苗，耐用的疫苗和耐用的体液和细胞免疫反应，以造成家禽行业的严重经济损失。对于不靶向的疫苗，靶向疫苗的疫苗却显着，这些研究显着，这些疫苗是明显的。 MSD动物健康“通过这种合作，我们将调查我们开发的目标季苯基输送疫苗（TADV）如何进一步优化oi）oi）诱导更快，并为对地理区域的抗原感染家禽提供更有效和更广泛的免疫反应II）鸡的生产和递送方法是通过良好的昆虫培养物和重组载体（土耳其的Herpesv irus）系统。进口和人类病原体。