STRUCTURE AND FUNCTION OF VISUAL CORTEX

视觉皮层的结构和功能

基本信息

批准号：
3260582
负责人：
RICK C.S. LIN
金额：
$ 10.93万
依托单位：
DUKE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1981
资助国家：
美国
起止时间：
1981-07-01 至 1986-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3260582
关键词：
axon dendrites efferent nerve electrophysiology evoked potentials histology neuroanatomy neurophysiology

项目摘要

The main goal of the proposed research is to study the functional organization of visual cortex in the rat. First, we plan to identify the morphology of specific neuron populations which project to known extrinsic targets. We will continue to use a new modified technique (Fran et al. '80) of adding lysolecithin to horesradish peroxidase (HRP) and then ionto- phoretically injecting this mixture via a micropipette (tip sixe 10 Mum) with either ster- eotaxid and/or electrophysiologcal identification of appropriate brain regions. The detailed morphological classes of homogenously backfilled neurons of a particular pathway will be analyzed. In related experiments, collateral axonal projections will be studied by injecting suspected targets with two different fluorescent dyes. The potential results from these studies will set the stage for our functional analysis of neurons via intracellular recording and morphological identification. Second, we will continue the quantitative analysis of single neurons in terms of their receptive field properties by extracellular recording and their afferent and efferent connections determined by electrically stimulating known sources of inputs to the visual cortex such as the thalamus and contralateral cortex. Then the neuron will be peneatrated by the microlectrode, rechecked to ensure the identity of the neuron and injected with HRP. The HRP-filling reveals a detailed morphology including the dendritic and axonal branching patterns. An attempt will be made to correlate the dendritic and/or axonal arborization patterns to response properties such as orientation and direction selectivities, receptive field size, affarent and efferent connections and conduction velocity. In conclusion, we expect that these approaches will provide valuable insights and establish relationships between the neuronal morphology and physiological properties as well as known extrinsic projections.

拟议研究的主要目标是研究功能大鼠视觉皮层的组织。首先，我们计划确定特定神经元种群的形态，这些神经元群体投射到已知的外部外部目标。我们将继续使用新的修改技术（Fran等。 '80）将溶血素添加到Horesradish过氧化物酶（HRP）中，然后将离子添加通过微型移动物质地注入该混合物（TIP SIXE 10 MUM）用ster- eotaxid和/或电生理鉴定适当的大脑区域。详细的形态学类将分析特定途径的同质回填神经元。在相关实验中，侧支轴突投影将通过将可疑靶标注射两个不同的荧光染料。这这些研究的潜在结果将为我们的功能奠定阶段通过细胞内记录和形态学分析神经元鉴别。其次，我们将继续对单神经元在其接受场特性方面细胞外记录及其传入和传出连接通过电气刺激已知的输入来源来确定视觉皮层，例如丘脑和对侧皮层。然后神经元将被微层的修剪，重新检查以确保神经元的身份并注入HRP。 HRP填充显示详细的形态，包括树突状和轴突分支模式。尝试将树突状和/或轴突关联对响应属性的树木化模式，例如方向和方向选择性，接受场的大小，副本和辐射连接和传导速度。总之，我们期望这些方法将提供宝贵的见解并建立关系在神经元的形态和生理特性以及已知的外部预测。