Function and regulation of action potential bursts in the auditory system.

听觉系统动作电位爆发的功能和调节。

• 批准号: 8465754
• 负责人: Kevin J Bender
• 金额: $ 18.89万
• 依托单位: ERNEST GALLO CLINIC AND RESEARCH CENTER
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-12-01 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8465754
• 关键词: Action Potentials; Affect; Auditory; Auditory system; Axon; Back; Brain Stem; Brain region; Calcium Channel; Cells; Coupled; Cues; Data; Dendrites; Dopamine; Efferent Neurons; Electrophysiology (science); Ensure; Epilepsy; Failure; Fire - disasters; Frequencies; Functional disorder; Fusiform Cell; Generations; Goals; Image; Imaging Techniques; Learning; Mediating; Membrane; Mentors; Neurons; Noise; Optics; Output; Pathway interactions; Pharmacology; Phase; Phosphorylation; Photons; Play; Process; Regulation; Role; Second Messenger Systems; Shapes; Signal Pathway; Signal Transduction; Site; Sound Localization; Structure; Synapses; Synaptic plasticity; Techniques; Testing; Time; Tinnitus; Whole-Cell Recordings; Work; dorsal cochlear nucleus; multisensory; nerve supply; neuronal excitability; novel; postsynaptic; presynaptic; second messenger; sensor; sound; voltage

Project summary: Circuits in the auditory brainstem dorsal cochlear nucleus (DCN) are believed to aid in sound localization in the vertical plane using monaural cues. Moreover, DCN circuitry integrates auditory and non-auditory inputs to aid in orientation toward sounds or to suppress self-generated noise, thereby increasing the salience of external sounds. Our long-term goal is to understand the synaptic mechanisms that contribute to these functions. Multisensory integration is controlled, in part, by high-frequency action potential bursts from inhibitory cartwheel cells; yet the way in which bursts are generated, and the effects of burst inhibition on postsynaptic integration, remain unclear. The objective of this proposal is to define mechanisms governing burst generation, focusing on the role of newly-discovered low-threshold activated calcium channels localized to the site of action potential initiation in the axon initial segment. First, we will use a combination of electrophysiology and 2-photon imaging to identify signaling pathways that control neuronal output by regulating initial segment calcium channel activity. Second, we will take advantage of novel voltage imaging techniques to determine how calcium channels contribute to the generation of bursts in the initial segment. Finally, we will determine how inhibitory synaptic input affects integration in the efferent neurons of the DCN, fusiform cells, contrasting the effects of single action potentials and bursts.

项目摘要：据信听觉脑干背部耳蜗核（DCN）中的电路可以帮助 使用单膜提示在垂直平面中的声音定位。此外，DCN电路集成了听觉和 非审计输入以帮助朝向声音或抑制自我生成的噪声，从而增加 外部声音的显着性。我们的长期目标是了解贡献的突触机制 这些功能。多感官集成部分受到高频动作电势爆发的控制 抑制性车轮细胞；然而，产生爆发的方式以及爆发抑制的影响 突触后整合，尚不清楚。该提议的目的是定义管理机制 爆发产生，重点是新发现的低阈值激活的钙通道的作用 到轴突初始段中的动作电位启动位置。首先，我们将使用 电生理学和2光子成像，以鉴定通过控制神经元输出的信号通路 调节初始段钙通道活性。其次，我们将利用新颖的电压成像 确定钙通道如何促进初始段中突发产生的技术。 最后，我们将确定抑制性突触输入如何影响DCN的传出神经元中的整合 梭形细胞，与单个动作电位和爆发的影响对比。