Enhancing cobalamin (vitamin B12) bioavailability in culturally appropriate foods in India

提高印度文化适宜食品中钴胺素（维生素 B12）的生物利用度

• 批准号: BB/S014020/1
• 负责人: Martin Warren
• 金额: $ 192.65万
• 依托单位: University of Kent
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FS014020%2F1
• 关键词: Enhancing; cobalamin; vitamin; B12; bioavailability

B12 deficiency and insufficiency is thought to represent a hidden worldwide epidemic of nutrient malnutrition in both developed and developing countries. However, there is no doubt that in India the problem is on a vast scale, with estimates of around 1 billion people having low levels of the nutrient. This is a consequence not only of general malnutrition but also due to cultural practices associated with vegetarianism, since crops have very low levels of B12. In this application we aim to initiate a programme of work to address this nutrient-deficiency challenge, by combining expertise in the UK with that found in India. Specifically, we want to investigate if a newly constructed recombinant strain of E. coli can be used to provide large quantities of purified B12. To achieve this we will combine the metabolic engineering work at the University of Kent with the process engineering capability found at the ICT in Mumbai. The development of a suitable extraction procedure will allow us to interact with chemical companies such as Cipla, who have expressed an interest in taking this forward if a suitable high-yielding method is developed. This holds the potential for India to be able to produce all its own B12 rather than having to rely on expensive imports. In so doing, this will also address serious concerns about global nutrient supply, since currently around 90% of B12 is produced in China. We also plan to explore the use of alternative culturally acceptable enriched sources of B12 in the form of algae and higher plants. Many algae are known to be able to absorb the nutrient but only limited studies have been undertaken in order to assess whether the B12 found in algae is bioavailable to humans. Similarly, we have recently found that some higher plants, such as spring cress, are also able to absorb B12 when the plants are grown with the nutrient. However, as with algae, it is not known whether the nutrient is bioavailable. Therefore, apart from investigating ways to enhance B12 uptake into algae and higher plants, we will undertake volunteer trials to determine whether food prepared from these sources is able to alleviate B12 deficiency in the volunteers. This work will combine expertise in the UK in providing the growth conditions, the food technologists in Mumbai and the clinical expertise in Pune to address this problem. This represents an exciting series of experiments that holds the potential to provide transformative solutions to B12 deficiency in both developing developed countries.

B12缺乏症和不足是发达国家和发展中国家中隐藏的养分营养不良流行。但是，毫无疑问，在印度，这个问题是广泛的，估计有大约10亿人的营养水平。这不仅是一般营养不良的结果，而且是由于与素食主义相关的文化实践所致，因为农作物的B12水平很低。在此应用程序中，我们旨在通过将英国的专业知识与印度发现的专业知识相结合，以启动一项工作，以应对这种营养缺陷挑战。具体而言，我们要研究新构建的大肠杆菌重组菌株是否可用于提供大量纯化的B12。为了实现这一目标，我们将将肯特大学的代谢工程工作与孟买ICT的工程工程能力相结合。合适的提取程序的开发将使我们能够与Cipla等化学公司进行互动，Cipla表示，如果开发了合适​​的高产方法，他们表示有兴趣将其前进。这有可能使印度能够生产所有自己的B12，而不必依靠昂贵的进口。这样一来，这也将解决对全球养分供应的严重关注，因为目前在中国生产的B12中约有90％。我们还计划探索以藻类和较高植物形式的替代文化可接受的B12来源的使用。已知许多藻类能够吸收养分，但仅进行了有限的研究，以评估人类在藻类中发现的B12是否是生物利用的。同样，我们最近发现，当植物用养分生长时，一些较高的植物（例如春季晶体）也能够吸收B12。但是，与藻类一样，尚不清楚养分是否可生物利用。因此，除了调查增强B12吸收藻类和较高植物的方法外，我们还将进行志愿者试验，以确定从这些来源准备的食物是否能够减轻志愿者的B12缺乏症。这项工作将结合英国提供增长条件的专业知识，孟买的食品技术人员以及浦那的临床专业知识，以解决这一问题。这代表了一系列令人兴奋的实验，该实验具有为两个发展中国家的B12缺乏症提供变革性解决方案的潜力。

项目成果

Metalation calculators for E. coli strain JM109 (DE3): aerobic, anaerobic, and hydrogen peroxide exposed cells cultured in LB media.

• DOI: 10.1093/mtomcs/mfac058
• 发表时间: 2022-09-01
• 期刊: METALLOMICS
• 影响因子: 3.4
• 作者: Foster, Andrew W.;Clough, Sophie E.;Aki, Zeynep;Young, Tessa R.;Clarke, Alison R.;Robinson, Nigel J.
• 通讯作者: Robinson, Nigel J.

Exploring the onset of B12-based mutualisms using a recently evolved Chlamydomonas auxotroph and B12-producing bacteria

使用最近进化的营养缺陷型衣藻和生产 B12 的细菌探索基于 B12 的互利共生的开始

• DOI: 10.17863/cam.84192
• 发表时间: 2022
• 作者: Bunbury F

Modification of bacterial microcompartments with target biomolecules via post-translational SpyTagging.

• DOI: 10.1039/d3ma00071k
• 发表时间: 2023-07-17
• 期刊: Materials advances
• 影响因子: 5

Metalation calculators for E. coli strain JM109 (DE3): Aerobic, anaerobic and hydrogen peroxide exposed cells cultured in LB media

大肠杆菌菌株 JM109 (DE3) 的金属化计算器：在 LB 培养基中培养的需氧、厌氧和过氧化氢暴露细胞

• DOI: 10.1101/2022.05.06.490408
• 发表时间: 2022
• 作者: Foster A