A sustainable production platform for industrially relevant phytochemicals

工业相关植物化学品的可持续生产平台

基本信息

批准号：
BB/R017603/1
负责人：
Gary Loake
金额：
$ 26.24万
依托单位：
University of Edinburgh
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2018
资助国家：
英国
起止时间：
2018 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FR017603%2F1
关键词：
sustainable production platform industrially relevant

项目摘要

A clinical trial run by an established UK company has identified two natural plant molecules that inhibit skin inflammation. Unfortunately, the amount of these molecules from the source plant is very low and therefore extraction from the source plant is not feasible on an industrial scale. A potential solution to this problem is to use cultured plant cells, which can been grown on a large scale in multi-tonne bioreactors and optimised for the production of these target plant natural products. Significantly, by employing non-GM genetic approaches, the yield of these target molecules will be significantly increased in these cultured plant cells. This approach will also enable new insights into the biochemical regulation of these molecules within the source plant, potentially leading to further improvements in yield. The target natural products isolated from the generated plant cell lines will also be tested and compared to the same molecules extracted from the source plant in a large number of biomedical-based assays. It is anticipated this work will show the molecules produced from the generated plant cell lines are functionally equivalent to those extracted from the source plant. Once confirmed, and beyond the scale of this particular research project, the company will scale-up the growth of the generated plant cells and isolate the target molecules for their introduction into commercial products.

由一家既定的英国公司开展的临床试验确定了两个抑制皮肤炎症的天然植物分子。不幸的是，来自源工厂的这些分子的量非常低，因此从工业规模上提取了源工厂不可行。解决此问题的一种潜在解决方案是使用培养的植物细胞，该细胞可以在多吨生物反应器中大规模生长，并为生产这些靶植物天然产物的生产而进行了优化。值得注意的是，通过采用非GM遗传方法，在这些培养的植物细胞中，这些靶分子的产率将显着增加。这种方法还将能够对源工厂内这些分子的生化调节进行新的见解，从而有可能进一步提高产量。从生成的植物细胞系中分离出的目标天然产物也将进行测试，并将其与从源工厂中从源工厂中提取的相同分子进行比较。预计这项工作将表明从产生的植物细胞系产生的分子在功能上等同于从源工厂中提取的分子。一旦得到确认，并且超出了该特定研究项目的规模，该公司将扩大生成的植物细胞的生长，并将目标分子隔离到商业产品中。