NABOR: A Sustainable, High-quality Neuroscience Antibody Open Resource

NABOR：可持续、高质量的神经科学抗体开放资源

• 批准号: 10406234
• 负责人: Melina Fan
• 金额: $ 134.03万
• 依托单位: ADDGENE, INC.
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-06-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10406234
• 关键词: Affinity; Aliquot; Antibodies; Antibody Affinity; Antibody Formation; Award; Awareness; BRAIN initiative; Biological Assay; Brain; California; Catalogs; Collection; Communities; Computer software; Data; Deposition; Doctor of Philosophy; Education and Outreach; Educational workshop; Future; Goals; Grant; Growth; Incentives; Infrastructure; Institutes; Laboratories; Libraries; Massachusetts; Measures; Modeling; Molecular; Monoclonal Antibodies; Neurosciences; Neurosciences Research; Online Systems; Outcome; Performance; Plasmids; Production; Proteins; Protocols documentation; Publications; Quality Control; Reagent; Recombinant Antibody; Recombinants; Research Personnel; Resources; Scientist; Services; Ships; Technology; United States National Institutes of Health; Universities; Validation; Viral; Viral Vector; Work; base; crowdsourcing; data hub; data sharing; digital; education resources; empowered; experience; nanobodies; neural circuit; outreach; sample collection; sharing platform; tool

PROJECT SUMMARY The goal of this project is to build an open-access recombinant antibody/affinity reagent resource for the neuroscience community called NABOR (Neuroscience AntiBody Open Resource). Reliable access to affinity reagents validated for use in diverse neuroscience research applications is crucial to accomplishing numerous goals of the BRAIN Initiative. Currently available affinity reagents from companies can be variable in quality and the molecular identity of the material is completely unknown to scientists. Scientists are now creating recombinant, plasmid-based affinity reagents such as recombinant monoclonal antibodies (R-mAbs), Single Chain Variable Fragments (ScFVs) and nanobodies (nAbs). Addgene, a nonprofit company, is a leader in plasmid storage, quality control, and distribution. Addgene will create NABOR as an open-access library of these plasmids and will provide them in ready-to-use protein format, along with protocols and educational guides. Aim 1 is to build a high-quality production and distribution pipeline for ready-to-use recombinant affinity reagents. Two leading BRAIN Initiative investigators, James Trimmer, Ph.D., of the University of California Davis, and Kwanghun Chung, Ph.D., of the Massachusetts Institute of Technology, will kick-start NABOR by contributing their extensive collections of neuroscience affinity reagents to this collection. Each lot will be subjected to rigorous quality control, initially in Dr. Trimmer’s laboratory and later at Addgene. Aim 2 is to build an open-access community resource platform for data sharing. This will include a web-based Data Hub to crowdsource reagent validation in a variety of applications. Aim 3 is to execute an education and outreach strategy that raises awareness of existing tools and solicits new tools. Addgene will share protocols and educational guides to make scientists successful when using the reagents and work directly with the community to create the NABOR catalog needed for neural circuit mapping and our understanding brain function. NABOR reagents and supporting information will be made available through Addgene’s existing channels for production, distribution and technical support. Successful completion of this project will result in enhanced visibility, access, and value for affinity reagents created by the BRAIN Initiative.

项目摘要 该项目的目的是建立开放式重组抗体/亲和力试剂资源 神经科学界称为Nabor（神经科学抗体开放资源）。可靠访问亲和力 用于在潜水神经科学研究应用中验证的试剂对于完成许多人至关重要 大脑计划的目标。当前可用的公司的亲和力试剂可能是质量可变的 并且材料的分子身份完全是科学家所不知道的。科学家现在正在创造 重组，基于质粒的亲和力试剂，例如重组单克隆抗体（R-MAB），单一抗体 链可变片段（SCFV）和纳米构造（NABS）。 非营利性公司Addgene是质粒存储，质量控制和分销的领导者。 Addgene会 创建Nabor作为这些质粒的开放访问库，并将其提供以现成的蛋白质 格式，以及协议和教育指南。目标1是建立高质量的生产和分销 馈线可用来使用重组亲和力试剂。两个领先的大脑倡议调查员詹姆斯 加利福尼亚大学戴维斯大学的Trimmer博士和马萨诸塞州的Kwanghun Chung博士 技术研究院将通过贡献其广泛的神经科学亲和力来启动纳博 该系列的试剂。每个批次都将受到严格的质量控制，最初是在Trimmer博士的 实验室，后来在Addgene。 AIM 2是为数据构建一个开放访问社区资源平台 分享。这将包括一个基于Web的数据枢纽，以在各种应用程序中进行众包试剂验证。 AIM 3是执行一种教育和外展策略，以提高人们对现有工具的认识 工具。 Addgene将共享协议和教育指南，以使科学家在使用时成功 试剂并直接与社区合作，以创建神经电路映射所需的Nabor目录 以及我们的大脑功能。 Nabor试剂和支持信息将通过Addgene的现有渠道提供 生产，分销和技术支持。成功完成该项目将导致增强 大脑计划创建的亲和力试剂的可见性，访问和价值。