BBSRC-Embrapa Genomics and pathogenomics to improve FHB and brusone resistance in Brazilian wheat

BBSRC-Embrapa 基因组学和病原学可提高巴西小麦的 FHB 和 brusone 抗性

• 批准号: BB/N004442/1
• 负责人: Paul Nicholson
• 金额: $ 9.25万
• 依托单位: John Innes Centre
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FN004442%2F1
• 关键词: BBSRC; Embrapa; Genomics; pathogenomics; improve

Project teamThe project team combines research leaders with complementary expertise in both the wheat host and the FHB and brusone fungal pathogens in Brazil and the UK. Pedro Scheeren (Embrapa) and Eduardo Caierao (Embrapa) have unrivalled knowledge of Brazilian wheat production, providing expertise in wheat breeding alongside knowledge of germplasm resistance status that is essential for genome wide association scans to be undertaken to identify genomic regions associated with resistance to FHB and brusone. Cristobal Uauy (JIC) is internationally recognised for his expertise in wheat genetics and gene cloning in cereals. CU collaborates closely with Ksenia Krasileva (TGAC/TSL) who leads the development of functional genomics tools in wheat, including the development of exome-capture platforms that will form one of the pillars of the project. Paul Nicholson (JIC) leads a research group investigating the genetic basis of resistance to FHB in cereals and will contribute to both host and pathogen components in the project in partnership with Flávio Santana and Maria Imaculada Moreira Lima (Embrapa), who lead research on FHB resistance. James Cockram (NIAB) has extensive experience in combining molecular genetics and genomics with statistical techniques to investigate complex traits in wheat. These skills will be used in collaboration with Luciano Consoli (Embrapa) who leads the quantitative genetics programme, to undertake association mapping of target traits in Brazilian wheat germplasm. James Cockram will provide training to Embrapa personnel to enable them to undertake additional analyses on FHB and brusone data as these become available.Joao Leodato (Embrapa) is a recognised expert on brusone disease and will work alongside Gisele Torres (Embrapa) who has extensive experience of working with the Brusone pathogen and interactions with the wheat host using functional genomics analysis. Antonio Nhani Jr (Embrapa) employs genomics to investigate virulence and host specificity in the brusone pathogen. The skills and knowledge provided by Embrapa researchers will be complemented those of Nick Talbot (University of Exeter) who is internationally recognised for his expertise in the genetics and virulence of the brusone fungus. Diane Saunders (TGAC/JIC) leads research into pathogen populations and virulence, providing essential insight into the structure of the populations of the FHB and brusone pathogens that will be utilised within studies of the genetics of host resistance. Resources and InfrastructureIn addition to the highly complementary skill sets of the Embrapa and UK participants, the organisations contribute complementary resources and infrastructure. Embrapa has excellent field trials facilities and trained personnel across Brazil in regions where the environment is most conducive to the FHB and brusone diseases enabling them to undertake high quality disease trials. The Embrapa site at Passo Fundo is exceptionally well equipped with laboratory, computing infrastructure, glasshouse and plant growth facilities in an environment that enables the rapid growth of plants to minimise generation time and ensure efficient development of plant populations to the required level of fixation for genotyping and phenotyping purposes. The UK partners within this project (JIC, TGAC, NIAB and Exeter) all have extensive laboratory, computational and plant growth facilities, required for the host and pathogen genetics and genomics components of the project. In addition, TGAC has state of the art genome sequencing and transcriptional profiling facilities and computer hardware and software to undertake the associated data processing and analysis.

项目团队该项目团队由研究领导者组成，他们在巴西和英国的小麦宿主以及 FHB 和 brusone 真菌病原体方面具有互补的专业知识。小麦育种以及种质抗性状态的知识对于进行全基因组关联扫描以确定与 FHB 和 Cristobal Uauy 抗性相关的基因组区域至关重要。 CU 与 Ksenia Krasileva (TGAC/TSL) 密切合作，后者领导了小麦功能基因组学工具的开发，包括外显子组捕获平台的开发。 Paul Nicholson (JIC) 领导一个研究小组，调查谷物中 FHB 抗性的遗传基础，并将与该项目合作，为该项目的宿主和病原体成分​​做出贡献。领导 FHB 抗性研究的 Flávio Santana 和 Maria Imaculada Moreira Lima 在将分子遗传学和基因组学与统计技术相结合以研究小麦的复杂性状方面拥有丰富的经验。 Luciano Consoli (Embrapa) 负责领导巴西小麦种质目标性状的关联绘图，James Cockram 将为 Embrapa 的其他人员提供培训，使他们能够对 FHB 进行分析。 Joao Leodato (Embrapa) 是布鲁松病方面公认的专家，他将与 Gisele Torres (Embrapa) 一起工作，后者在处理布鲁松病原体以及使用功能基因组学分析与小麦宿主相互作用方面拥有丰富的经验。 Antonio Nhani Jr (Embrapa) 利用基因组学来研究布鲁松病原体的毒力和宿主特异性。Embrapa 研究人员提供的技能和知识将是。尼克·塔尔博特 (Nick Talbot)（埃克塞特大学）因其在布鲁松真菌遗传学和毒力方面的专业知识而获得国际认可，黛安·桑德斯 (TGAC/JIC) 领导了病原体种群和毒力的研究，为病原体的结构提供了重要的见解。 FHB 和 bruson 病原体种群将用于宿主抗性遗传学研究 资源和基础设施 除了 Embrapa 和 的高度互补的技能集之外。英国参与者、Embrapa 组织提供了互补的资源和基础设施，在巴西各地环境最有利于 FHB 和 bruson 疾病的地区拥有优秀的现场试验设施和训练有素的人员，使他们能够在帕索进行高质量的疾病试验。 Fundo 配备了非常齐全的实验室、计算基础设施、温室和植物生长设施，其环境能够使植物快速生长，从而最大限度地缩短世代时间，并确保植物种群的有效发展达到基因分型和固定所需的水平。该项目的英国合作伙伴（JIC、TGAC、NIAB 和 Exeter）均拥有该项目的宿主和病原体遗传学和基因组学组件所需的广泛的实验室、计算和植物生长设施。先进的基因组测序和转录分析设施以及计算机硬件和软件来进行相关的数据处理和分析。

项目成果

Type II Fusarium head blight susceptibility conferred by a region on wheat chromosome 4D.

小麦染色体 4D 上的一个区域赋予 II 型赤霉病易感性。

• DOI: http://dx.10.1093/jxb/eraa226
• 发表时间: 2020
• 期刊: Journal of experimental botany
• 影响因子: 6.9
• 作者: Hales B

A wheat kinase and immune receptor form host-specificity barriers against the blast fungus.

小麦激酶和免疫受体形成针对稻瘟菌的宿主特异性屏障。

• DOI: http://dx.10.1038/s41477-023-01357-5
• 发表时间: 2023
• 期刊: Nature plants
• 影响因子: 18
• 作者: Arora S

Dissecting the genetic basis of wheat blast resistance in the Brazilian wheat cultivar BR 18-Terena.

剖析巴西小麦品种 BR 18-Terena 抗稻瘟病的遗传基础。

• DOI: http://dx.10.1186/s12870-020-02592-0
• 发表时间: 2020
• 期刊: BMC plant biology
• 影响因子: 5.3
• 作者: Goddard R

Tissue-specific transcriptome responses to Fusarium head blight and Fusarium root rot.

对镰刀菌赤霉病和镰刀菌根腐病的组织特异性转录组反应。

• DOI: http://dx.10.3389/fpls.2022.1025161
• 发表时间: 2022
• 期刊: Frontiers in plant science
• 作者: Haidoulis JF

Speed breeding in growth chambers and glasshouses for crop breeding and model plant research.

在生长室和温室中快速育种，用于作物育种和模型植物研究。

• DOI: http://dx.10.1038/s41596-018-0072-z
• 发表时间: 2018
• 期刊: Nature protocols
• 影响因子: 14.8
• 作者: Ghosh S