LABIAL SALIVARY GLANDS-AUTONOMIC CHARACTERIZATION

唇唾液腺自主特征

基本信息

批准号：
3223088
负责人：
Kenneth T Izutsu
金额：
$ 12.56万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
1991
资助国家：
美国
起止时间：
1991-09-30 至 1994-09-29
项目状态：
已结题

项目摘要

The overall objective of this work is to elucidate the autonomic regulation of human labial gland function. This proposal concentrates on characterizing the autonomic receptors present in the gland, and on documenting some of the biochemical and physiological responses evoked by receptor activation. The biochemical responses of present interest involve changes in gland cyclic nucleotide concentrations, while the physiological responses involve activation of cell K release. The specific aims are as follows: Specific Aim 1. To show that labial glands possess normal numbers of muscarinic cholinergic and beta-adrenergic receptors, and relatively low numbers of alpha-adrenergic receptors. Specific Aim 2. To determine the binding affinities of these receptors for the corresponding agonists and antagonists, and to obtain information regarding the receptor subtypes. Specific Aim 3. To characterize the biochemical and physiological effects of autonomic receptor activation in labial glands. The specific aims dealing with numbers of receptors and receptor binding affinities will be accomplished using a labial gland membrane preparation that will be incubated with appropriate radioligands in the presence or absence of various antagonists. The specific aim dealing with biochemical and physiological effects of autonomic receptor activation will involve incubating labial gland slices in vitro, and measuring receptor induced changes in gland cyclic nucleotide concentrations, or receptor induced activation of gland K release, a measure of gland electrolyte and, presumably, gland fluid secretion. Preliminary results are presented for all aspects of the work and show that the proposed experiments are feasible for labial gland samples. Completion of this work will lead to a significant increase in our knowledge of the normal autonomic regulation of human labial gland secretory function. The data and procedures developed in this study should find numerous applications in future studies in human salivary gland disease.

这项工作的总体目的是阐明自主法规人类唇腺功能。该建议集中于表征腺体中存在的自主受体和记录一些生化和生理反应受体激活。当前兴趣的生化反应涉及腺环核苷酸浓度的变化，而生理反应涉及细胞K释放的激活。具体目的是以下内容：特定目的1。表明唇腺具有正常数的毒蕈碱胆碱能和β-肾上腺素能受体，相对较低 α-肾上腺素受体的数量。具体目的2。确定这些受体的结合亲和力相应的激动剂和拮抗剂，并获得信息关于受体亚型。特定目的3。表征生化和生理效应唇腺中的自主受体激活。处理受体数量和受体结合数量的具体目的使用唇腺膜制备将实现亲和力在存在的情况下将与适当的放射线孵育或缺乏各种拮抗剂。处理生化的特定目的自主受体激活的生理影响将涉及在体外孵育唇腺切片，并测量受体诱导腺环核苷酸浓度或受体诱导的变化腺体K释放的激活，腺电解质的量度和大概是腺体液体分泌。提出了初步结果工作的所有方面，并表明提出的实验是可行的用于唇腺样品。这项工作的完成将导致我们的大幅增长了解人类唇腺正常自主法规分泌功能。本研究中开发的数据和程序应在未来的人类唾液腺中找到许多应用疾病。