LABIAL SALIVARY GLANDS-AUTONOMIC CHARACTERIZATION

唇唾液腺自主特征

基本信息

批准号：
3223088
负责人：
Kenneth T Izutsu
金额：
$ 12.56万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
1991
资助国家：
美国
起止时间：
1991-09-30 至 1994-09-29
项目状态：
已结题

项目摘要

The overall objective of this work is to elucidate the autonomic regulation of human labial gland function. This proposal concentrates on characterizing the autonomic receptors present in the gland, and on documenting some of the biochemical and physiological responses evoked by receptor activation. The biochemical responses of present interest involve changes in gland cyclic nucleotide concentrations, while the physiological responses involve activation of cell K release. The specific aims are as follows: Specific Aim 1. To show that labial glands possess normal numbers of muscarinic cholinergic and beta-adrenergic receptors, and relatively low numbers of alpha-adrenergic receptors. Specific Aim 2. To determine the binding affinities of these receptors for the corresponding agonists and antagonists, and to obtain information regarding the receptor subtypes. Specific Aim 3. To characterize the biochemical and physiological effects of autonomic receptor activation in labial glands. The specific aims dealing with numbers of receptors and receptor binding affinities will be accomplished using a labial gland membrane preparation that will be incubated with appropriate radioligands in the presence or absence of various antagonists. The specific aim dealing with biochemical and physiological effects of autonomic receptor activation will involve incubating labial gland slices in vitro, and measuring receptor induced changes in gland cyclic nucleotide concentrations, or receptor induced activation of gland K release, a measure of gland electrolyte and, presumably, gland fluid secretion. Preliminary results are presented for all aspects of the work and show that the proposed experiments are feasible for labial gland samples. Completion of this work will lead to a significant increase in our knowledge of the normal autonomic regulation of human labial gland secretory function. The data and procedures developed in this study should find numerous applications in future studies in human salivary gland disease.

这项工作的总体目标是阐明自主调节影响人类唇腺的功能。该提案集中于表征腺体中存在的自主受体，以及记录引起的一些生化和生理反应受体激活。目前感兴趣的生化反应包括腺体环核苷酸浓度的变化，而生理反应涉及细胞 K 释放的激活。具体目标如下如下：具体目标 1. 证明唇腺具有正常数量毒蕈碱胆碱能和β-肾上腺素能受体，且相对较低 α-肾上腺素能受体的数量。具体目标 2. 确定这些受体的结合亲和力相应的激动剂和拮抗剂，并获取信息关于受体亚型。具体目标 3. 表征生化和生理效应唇腺自主神经受体激活的影响。涉及受体数量和受体结合的具体目标将使用唇腺膜制剂来完成亲和力将在存在或存在的情况下与适当的放射性配体一起孵育不存在各种拮抗剂。处理生化的具体目标自主神经受体激活的生理效应将涉及体外培养唇腺切片并测量受体诱导腺体环核苷酸浓度或受体诱导的变化腺体钾释放的激活，腺体电解质的测量，以及，推测是腺液分泌。初步结果如下：各个方面的工作并表明所提出的实验是可行的用于唇腺样本。这项工作的完成将导致我们的了解人体唇腺正常自主调节分泌功能。本研究中开发的数据和程序应在人类唾液腺的未来研究中发现大量应用疾病。