ION CHANNELS IN SALIVARY GLAND DUCT CELLS

唾液腺管细胞中的离子通道

• 批准号: 2130743
• 负责人: Kenneth T Izutsu
• 金额: $ 13.33万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-04-01 至 1995-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2130743
• 关键词: G protein; acinar cell; calcium channel blockers; calcium flux; calcium indicator; calmodulin; carbachol; chloride channels; clone cells; electrical conductance; electrical measurement; electrical potential; electrophysiology; inositol phosphates; membrane permeability; muscarinic receptor; oral health; oscillography; photolysis; potassium channel; protein kinase C; quinine; radiotracer; saliva; salivary glands; single cell analysis; tissue /cell culture; toxin; voltage /patch clamp

The goal of these studies is to identify the ionic currents that underlie salivary gland ductal function and to elucidate the intracellular factors that regulate these currents. The approach .Is to use the electrophysiological technique of patch clamping in order to complement and extend findings made using radiotracer techniques. The hypotheses to be tested are derived from results obtained in our preliminary studies, and from previous electrophysiological studies of salivary gland acinar cells, and generally test whether the same ionic currents and current regulating mechanisms present in acinar cells are also present in ductal cells. The studies utilize whole-cell current measurements from unstimulated and muscarinically stimulated HSG-PA tissue culture cells, and single channel current measurements on excised membrane patches from these cells. The whole-cell current studies allow analysis and characterization of resting cell membrane conduc- tances as well as effects of various intracellular agents involved in cell signaling such as G-proteins, IP3, Ca ion concentrations, protein kinase C and calmodulin. The single channel current studies will allow a definitive characterization of the potassium (and possibly the Cl) channels present in these cells.

这些研究的目的是确定离子 构成唾液腺导管功能的电流 阐明调节这些电流的细胞内因素。 方法是利用电生理贴片技术 夹紧以补充和扩展使用的发现 放射性示踪剂技术。 导出要检验的假设 根据我们的初步研究结果以及之前的研究结果 唾液腺腺泡细胞的电生理学研究，以及 一般测试离子电流和电流是否相同 腺泡细胞中存在的调节机制也存在于 导管细胞。 该研究利用全细胞电流测量 来自未刺激和毒蕈碱刺激的 HSG-PA 组织 培养细胞，并对切除的细胞进行单通道电流测量 这些细胞的膜片。 全细胞电流研究 允许分析和表征静息细胞膜传导 所涉及的各种细胞内药物的含量和作用 细胞信号传导，例如 G 蛋白、IP3、Ca 离子浓度， 蛋白激酶 C 和钙调蛋白。 单通道电流研究 将允许对钾（和 可能是这些细胞中存在的 Cl) 通道。

项目成果

Characteristics and regulation of a muscarinically activated K current in HSG-PA cells.

HSG-PA 细胞中毒蕈碱激活 K 电流的特征和调节。

• DOI: 10.1152/ajpcell.1994.266.1.c58
• 发表时间: 1994
• 期刊: The American journal of physiology
• 作者: Izutsu,KT;Fatherazi,S;Wellner,RB;Herrington,J;Belton,CM;Oda,D
• 通讯作者: Oda,D

A K+ conductance can be transiently activated in HSG-PA cells.

HSG-PA 细胞中的 K 电导可被瞬时激活。

• DOI: 10.1177/10454411930040032701
• 发表时间: 1993
• 期刊: Critical reviews in oral biology and medicine : an official publication of the American Association of Oral Biologists
• 作者: Izutsu,KT;Oda,D;Yang,J
• 通讯作者: Yang,J

Mycoplasma orale infection affects K+ and Cl- currents in the HSG salivary gland cell line.

口腔支原体感染影响HSG唾液腺细胞系中的K电流和Cl电流。

• DOI: 10.1007/bf02722962
• 发表时间: 1996
• 期刊: In vitro cellular & developmental biology. Animal
• 作者: Izutsu,KT;Fatherazi,S;Belton,CM;Oda,D;Cartwright,FD;Kenny,GE
• 通讯作者: Kenny,GE

Hypotonically activated chloride current in HSG cells.

HSG 细胞中低渗激活的氯电流。

• DOI: 10.1007/bf00234940
• 发表时间: 1994
• 期刊: The Journal of membrane biology
• 作者: Fatherazi,S;Izutsu,KT;Wellner,RB;Belton,CM
• 通讯作者: Belton,CM