MATURATION AND PLASTICITY IN THE OLFACTORY SYSTEM

嗅觉系统的成熟和可塑性

• 批准号: 3216626
• 负责人: PETER C BRUNJES
• 金额: $ 11.76万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-04-01 至 1995-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3216626
• 关键词: afferent nerve; autoradiography; brain metabolism; cell differentiation; cell population study; developmental neurobiology; glia; immunocytochemistry; laboratory rat; microglia; neural plasticity; neuroanatomy; neurochemistry; newborn animals; olfactory lobe; opossums; protein biosynthesis; stainings; synapses

The experiments proposed below are designed to examine development in the olfactory bulb and its principal synaptic targets, the anterior olfactory nucleus and primary olfactory cortex. The olfactory system has been chosen for several reasons. First, it is highly organized, facilitating quantitative examinations of early development and making subtle alterations relatively easy to discern. Many interesting forms of early plasticity have been demonstrated in the system, including inductive tissue interactions and substantial sensitivity to early stimutation. Indeed early unilateral odor deprivation has been repeatedly demonstrated to result in some of the largest experience-induced changes observed in the central nervous system. As a result the region affords considerable potential for examinations of the mechanisms by which environmental factors help to specify early growth. Two groups of experiments are proposed. The first continues our quantitative examination of early olfactory bulb development in the rat which emphasizes studies of how alterations in functional activity affect bulb growth. Specifically, we will examine: patterns of protein synthesis both in normally developing and deprived bulbs to examine the early cell biology of the olfactory system changes in the development of bulb glia populations. Another set of studies examines early bulb growth in the gray, short-tailed opossum Monodelphis domestics. Monodelphis pups are born in a very immature state, and are quite accessible to early experimental manipulation allowing in depth examinations of early tissue interactions. Specific experiments include: examinations of early functional deprivation to gauge how activity influences early growth. assessment of the effects of bulb removal on the development of higher order olfactory structures. In summary, the proposed research will explore early developmental processes such as the instructive interactions which occur between tissues and the role of afferent activity in growth and differentiation.

下面提出的实验旨在检查 嗅球及其主要突触靶标，前嗅觉 细胞核和原发性皮层。 选择了嗅觉系统 由于几个原因。首先，它是有条理的，促进了 对早期发展的定量考试，并使微妙 改动相对容易辨别。许多有趣的早期形式 在系统中已经证明了可塑性，包括电感组织 相互作用和对早期刺激的敏感性。确实很早 单方面的气味剥夺已被反复证明导致 中央中观察到的一些最大的经验引起的变化 神经系统。 结果，该地区具有很大的潜力 检查环境因素有助于的机制 指定早期增长。 提出了两组实验。第一个继续我们 大鼠早期嗅球发育的定量检查 这强调了对功能活动变化如何影响的研究 灯泡生长。具体来说，我们将研究： 蛋白质合成的模式在正常发展和剥夺中 检查嗅觉系统的早期细胞生物学 鳞茎神经胶质种群的发展变化。 另一项研究检查了灰色，短尾的早期鳞茎生长 负鼠单纤维植物。幼崽诞生于一个非常不成熟的 状态，并且可以通过早期的实验操作来访问 在早期组织相互作用的深度检查中。具体实验 包括： 检查早期功能剥夺以衡量活动的考试 影响早期增长。 评估灯泡去除对较高发展的影响 订购嗅觉结构。总而言之，拟议的研究将探索 早期的发展过程，例如具有指导性的互动 发生在组织之间以及传入活性在生长中的作用 分化。