India-UK Nitrogen Fixation Centre (IUNFC)

印度-英国固氮中心 (IUNFC)

基本信息

批准号：
BB/N013387/1
负责人：
Philip Poole
金额：
$ 172.9万
依托单位：
University of Oxford
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2016
资助国家：
英国
起止时间：
2016 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FN013387%2F1
关键词：
India UK Nitrogen Fixation Centre

项目摘要

Nitrogen is an essential element of biological molecules and life on earth. Lack of usable nitrogen limits growth of microbes, plants, and animals. Lack of nitrogen in agricultural soils limits plant production of food, feed, fibre and fuel. Nature solved the nitrogen limitation problem via evolution of biological nitrogen fixation (BNF) in diazotrophic bacteria that reduce atmospheric N2 to NH3, which is readily assimilated into biological molecules. Biological nitrogen fixation is catalyzed by a complex metalloenzyme called nitrogenase whose oxygen-sensitivity may explain its restricted distribution amongst prokaryotes. Some plants, including most legumes and a few non-legumes form intimate, nitrogen-fixing symbioses with diazotrophs that provide the plants with ammonia. As a consequence, legumes have been an integral part of sustainable agricultural systems for thousands of years. However, in an effort to increase plant yields large-scale use of industrially-produced N-fertilizer has doubled the influx of N into the terrestrial biogeochemical N-cycle, with serious negative consequences for human health and the natural environment. Therefore, the long-term sustainability of massive N-fertilizer inputs in agriculture has come into question. A team of eight investigators in India and two in the UK have come together to enhance the use of legumes, increase their effectiveness at fixing nitrogen and develop endophytic diazotrophs for use on cereals such as rice. Our focus is on pigeon pea, which is the second most widely planted legume in India but suffers from poor rates of BNF and low yields. We will select elite lines of pigeon pea and competitive rhizobial inoculants to maximise yields while improving their robustness. In addition we will develop phosphate solubilising strains of rhizobia as the supply of phosphorous often limits the rates on BNF and plant yield. Desirable traits for BNF and phosphate solubilisation will then be stacked into rhizobial inocula. A final long-term aim is the transfer of BNF to cereals, particularly rice, by the use of endophytic diazoptophs that can be inoculated onto cereals. This will be done both by selection of new endophytic diazotrophs and by manipulation of the control of BNF and ammonia secretion in elite strains. To achieve these aims we bring together the national leaders in India and the UK with expertise in bacterial and plant genetics, genomics, biochemistry, molecular and cell biology, physiology, synthetic biology combined with a deep knowledge of BNF in Indian soils and different regions. Improving BNF in our target plants will increase croyields for resource-poor farmers while decreasing the use and environmental-impact of industrial N-fertilizers.

氮是地球上生物分子和生命的重要元素。缺乏可用的氮限制了微生物，植物和动物的生长。农业土壤中缺乏氮限制了植物生产食物，饲料，纤维和燃料。自然界通过在重18酶营养细菌中生物氮固定（BNF）的进化来解决氮限制问题，从而将大气N2降低至NH3，这很容易被化合到生物分子中。生物氮固定是由一种称为氮酶的复杂金属酶催化的，其氧气敏感可以解释其在原核生物中的限制分布。一些植物，包括大多数豆类和一些非葡萄糖，形成了与重18zotrophs的亲密，固定氮的共生，可为植物提供氨。结果，数千年来，豆类一直是可持续农业系统不可或缺的一部分。但是，为了增加植物，产生了工业生产的N-肥料的大规模使用已将N涌入陆生生物地球化学N-Cycle，对人类健康和自然环境造成了严重的负面影响。因此，大规模的N-肥料投入在农业中的长期可持续性受到质疑。印度八名调查人员和英国两名组成的团队聚集在一起，以增强豆类的使用，提高固定氮的有效性，并开发内生重18zotrophs，用于谷物等谷物。我们的重点是鸽子豌豆，这是印度第二种较广泛的豆科植物，但BNF的速度较差，产量较低。我们将选择鸽子豌豆的精英系和竞争性根瘤菌接种剂，以最大程度地提高产量，同时提高其稳健性。此外，由于磷的供应通常会限制BNF和植物产量的速率，因此我们将发展根瘤菌的磷酸盐溶解菌株。 BNF和磷酸盐溶解度的理想性状将堆叠成根瘤菌接种物中。最终的长期目的是将BNF转移到谷物，尤其是大米，它通过使用可以接种到谷物上的内生无移tigoptophs。这既可以通过选择新的内生重18zotrophs，又可以通过对精英菌株中BNF和氨分泌的控制进行操纵来完成。为了实现这些目标，我们将印度和英国的国家领导者汇集在一起，具有细菌和植物遗传学，基因组学，生物化学，分子和细胞生物学，生理学，合成生物学的专业知识，并结合了印度土壤中BNF的深刻了解。改善目标工厂的BNF将增加对资源贫乏的农民的克洛伊菲尔德，同时减少工业N-肥料的使用和环境影响。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Poor Competitiveness of Bradyrhizobium in Pigeon Pea Root Colonization in Indian Soils.

DOI：
10.1128/mbio.00423-21
发表时间：
2021-08-31
期刊：
mBio
影响因子：
6.4
作者：
Chalasani D;Basu A;Pullabhotla SVSRN;Jorrin B;Neal AL;Poole PS;Podile AR;Tkacz A
通讯作者：
Tkacz A

Multimodal correlative imaging and modelling of phosphorus uptake from soil by hyphae of mycorrhizal fungi.

DOI：
10.1111/nph.17980
发表时间：
2022-04
期刊：
NEW PHYTOLOGIST
影响因子：
9.4
作者：
Keyes, Sam;van Veelen, Arjen;Fletcher, Dan McKay;Scotson, Callum;Koebernick, Nico;Petroselli, Chiara;Williams, Katherine;Ruiz, Siul;Cooper, Laura;Mayon, Robbie;Duncan, Simon;Dumont, Marc;Jakobsen, Iver;Oldroyd, Giles;Tkacz, Andrzej;Poole, Philip;Mosselmans, Fred;Borca, Camelia;Huthwelker, Thomas;Jones, David L.;Roose, Tiina
通讯作者：
Roose, Tiina

Disrupting hierarchical control of nitrogen fixation enables carbon-dependent regulation of ammonia excretion in soil diazotrophs.

DOI：
10.1371/journal.pgen.1009617
发表时间：
2021-06
期刊：
PLoS genetics
影响因子：
4.5
作者：
Bueno Batista M;Brett P;Appia-Ayme C;Wang YP;Dixon R
通讯作者：
Dixon R

Genomic Diversity of Pigeon Pea (Cajanus cajan L. Millsp.) Endosymbionts in India and Selection of Potential Strains for Use as Agricultural Inoculants.

DOI：
10.3389/fpls.2021.680981
发表时间：
2021
期刊：
Frontiers in plant science
影响因子：
5.6
作者：
Jorrin B;Maluk M;Atoliya N;Kumar SC;Chalasani D;Tkacz A;Singh P;Basu A;Pullabhotla SV;Kumar M;Mohanty SR;East AK;Ramachandran VK;James EK;Podile AR;Saxena AK;Rao D;Poole PS
通讯作者：
Poole PS

Role and Regulation of Poly-3-Hydroxybutyrate in Nitrogen Fixation in Azorhizobium caulinodans.

聚 3-羟基丁酸酯在 Azorhizobium caulinodans 固氮中的作用和调节。