14 ERA-CAPS: INvestigating TRiticeae EPIgenomes for Domestication (INTREPID)

14 ERA-CAPS：研究小麦科 EPI 基因组用于驯化 (INTREPID)

• 批准号: BB/N005104/1
• 负责人: Anthony Hall
• 金额: $ 54.17万
• 依托单位: University of Liverpool
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FN005104%2F1
• 关键词: 14; ERA; CAPS; INvestigating; TRiticeae

Plant breeding uses DNA sequence variation to make new allelic combinations for crop improvement. Our creation of the first wheat gene sequence assemblies (Brenchley et al Nature 491, 705) has enabled new levels of high throughput precise genotyping for breeding this globally important crop. Nevertheless, there are other levels of heritable variation, such as epigenetic modifications, that are widely thought to play a key role in shaping genomes and creating new variation. We have recently developed highly efficient re-sequencing technologies for wheat that can measure DNA methylation in genes of multiple lines. This provides an outstanding opportunity to assess epigenetic variation in a major polyploid crop and understand how it may influence traits. The overall objective of this proposal is to use newly available wheat genome resources, together with our innovative application of exome capture and bisulphite sequencing, to measure epigenetic modifications in wheat genes, and relate these to gene expression and the acquisition of new phenotypes, and how they may contribute to genetic changes such as gene loss during polyploid formation.

植物育种利用 DNA 序列变异来产生新的等位基因组合以改良作物。我们创建的第一个小麦基因序列组件（Brenchley 等人 Nature 491, 705）使高通量精确基因分型达到新水平，以培育这种全球重要的作物。然而，还有其他层面的可遗传变异，例如表观遗传修饰，人们普遍认为它们在塑造基因组和创造新变异方面发挥着关键作用。我们最近开发了高效的小麦重测序技术，可以测量多个品系基因中的 DNA 甲基化。这为评估主要多倍体作物的表观遗传变异并了解它如何影响性状提供了绝佳的机会。该提案的总体目标是利用新近可用的小麦基因组资源，结合外显子组捕获和亚硫酸氢盐测序的创新应用，测量小麦基因的表观遗传修饰，并将其与基因表达和新表型的获得联系起来，以及如何它们可能会导致遗传变化，例如多倍体形成过程中的基因丢失。

项目成果

Analysis of the recombination landscape of hexaploid bread wheat reveals genes controlling recombination and gene conversion frequency

六倍体面包小麦的重组景观分析揭示了控制重组和基因转换频率的基因

• DOI: 10.1101/539684
• 发表时间: 2019
• 作者: Gardiner L

Integrating genomic resources to present full gene and promoter capture probe sets for bread wheat

整合基因组资源，提供面包小麦的完整基因和启动子捕获探针组

• DOI: 10.1101/363663
• 发表时间: 2018
• 作者: Gardiner L

A genome-wide survey of DNA methylation in hexaploid wheat.

• DOI: 10.1186/s13059-015-0838-3
• 发表时间: 2015-12-10
• 期刊: Genome biology
• 影响因子: 12.3
• 作者: Gardiner LJ;Quinton-Tulloch M;Olohan L;Price J;Hall N;Hall A
• 通讯作者: Hall A

A framework for gene mapping in wheat demonstrated using the Yr7 yellow rust resistance gene

使用 Yr7 黄锈病抗性基因展示的小麦基因图谱框架

• DOI: 10.1101/793604
• 发表时间: 2019
• 作者: Gardiner L