14 ERA-CAPS Regulating Tomato quality through Expression (RegulaTomE)

14 ERA-CAPS 通过表达调节番茄质量 (RegulaTomE)

• 批准号: BB/N005023/1
• 负责人: Cathie Martin
• 金额: $ 78.9万
• 依托单位: John Innes Centre
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FN005023%2F1
• 关键词: 14; ERA; CAPS; Regulating; Tomato

The twin objectives of RegulaTomE are to determine the importance of transcriptional regulation of the metabolic pathways defining quality traits in tomato and to identify such transcriptional regulators at the molecular level. The selected quality traits include those determining antioxidant capacity which impacts shelf life and nutritional value as well as those determining fruit flavor and over-ripening which influence organoleptic properties and shelf-life. Loci contributing to abiotic stress tolerance will also be identified toward the combined goals of more nutritious, stable and sustainable crops. RegulaTomE will use the natural variation available in introgression lines (ILs) resulting from wild species crosses to tomato to assess the importance of transcriptional regulation, identify additional regulatory genes and assess underlying genetic and epigenetic variation. RegulaTomE will additionally assess the potential for direct or indirect use of natural variation from a largely untapped wild species resource for crop improvement. To identify genes regulating metabolic pathways using the Solanum lycopersicoides ILs, and to capture as much genetic and epigenetic variation as possible for application to gene discovery and tomato improvement, resources need to be developed, including a genome reference sequence for S. lycopersicoides and metabolite, DNA methylation and transcriptome profiles of IL fruit. Resulting data will be made available without restriction via existing public databases, providing invaluable resources for the community to exploit further the untapped natural variation in S.lycopersicoides. RegulaTomE will lead to regulatory gene identification (an important advance in terms of fundamental understanding), and provide new tools for metabolic engineering of fruit quality. More immediately, the natural variation in fruit quality revealed by the S.lycopersicoides ILs could be applied to tomato improvement either directly through introgression into cultivated varieties or indirectly through the identification of target loci and corresponding allelic variation making positive contributions to quality traits within S. lycopersicum breeding germplasm and in other close relatives of tomato. The cooperation of scientists from five major European organisations and two US Universities producing world-leading research on fleshy fruit and agricultural/horticultural innovation will allow the development of tools and resources on a scale unavailable at a national level. The outputs of RegulaTomE will provide a framework of understanding as well as tools, in the form of genes, target loci and molecular markers, to support development of longer shelf-life, more nutritious and more flavorsome fleshy fruits in other horticultural crops. The effectiveness of these resources and the significance of the knowledge acquired on RegulaTomE will ensure that the project contributes directly to food security and sustainable fruit cultivation.

RegulaTomE 的双重目标是确定定义番茄品质性状的代谢途径转录调控的重要性，并在分子水平上识别此类转录调控因子。选定的质量性状包括决定影响保质期和营养价值的抗氧化能力的质量性状，以及决定影响感官特性和保质期的水果风味和过度成熟的质量性状。还将确定有助于非生物胁迫耐受性的基因座，以实现更有营养、稳定和可持续作物的综合目标。 RegulaTomE 将利用野生物种与番茄杂交产生的基因渗入系 (IL) 中可用的自然变异来评估转录调控的重要性，识别其他调控基因并评估潜在的遗传和表观遗传变异。 RegulaTomE还将评估直接或间接利用未开发的野生物种资源的自然变异来改良作物的潜力。为了利用番茄 IL 识别调节代谢途径的基因，并捕获尽可能多的遗传和表观遗传变异以应用于基因发现和番茄改良，需要开发资源，包括番茄和代谢物的基因组参考序列， IL 果实的 DNA 甲基化和转录组谱。结果数据将通过现有公共数据库不受限制地提供，为社区进一步开发番茄未开发的自然变异提供宝贵的资源。 RegulaTomE 将导致调控基因识别（基本理解方面的重要进步），并为水果品质的代谢工程提供新工具。更直接的是，S.lycopersicoides IL 揭示的果实品质的自然变异可以直接通过渗入栽培品种或间接通过识别目标基因座和相应的等位基因变异应用于番茄改良，从而对 S.lycopersicoides 内的品质性状做出积极贡献。番茄育种种质和番茄的其他近亲。来自五个主要欧洲组织和两所美国大学的科学家的合作，在肉质水果和农业/园艺创新方面进行世界领先的研究，这将有助于开发国家层面上无法实现的大规模工具和资源。 RegulaTomE 的成果将以基因、目标位点和分子标记的形式提供理解框架和工具，以支持其他园艺作物开发更长保质期、更营养和更美味的肉质水果。这些资源的有效性以及在 RegulaTomE 上获得的知识的重要性将确保该项目直接为粮食安全和可持续水果种植做出贡献。

项目成果

Voices of biotech.

生物技术的声音。

• DOI: 10.1038/nbt.3502
• 发表时间: 2016
• 期刊: Nature biotechnology
• 影响因子: 46.9
• 作者: Amit I

Beyond Purple Tomatoes: Combined Strategies Targeting Anthocyanins to Generate Crimson, Magenta, and Indigo Fruit

• DOI: 10.3390/horticulturae7090327
• 发表时间: 2021-09-01
• 期刊: HORTICULTURAE
• 影响因子: 3.1
• 作者: Butelli, Eugenio;Bulling, Katharina;Martin, Cathie
• 通讯作者: Martin, Cathie

Diverting tyrosine: Data from untargeted metabolic analysis of tomato fruit accumulating L-DOPA.

• DOI: 10.1016/j.dib.2020.106678
• 发表时间: 2021-03
• 期刊: Data in brief
• 影响因子: 1.2
• 作者: Breitel D;Brett P;Alseekh S;Fernie AR;Butelli E;Martin C
• 通讯作者: Martin C

Metabolic engineering of tomato fruit enriched in L-DOPA.

• DOI: 10.1016/j.ymben.2020.11.011
• 发表时间: 2021-05
• 期刊: Metabolic engineering
• 影响因子: 8.4
• 作者: Breitel D;Brett P;Alseekh S;Fernie AR;Butelli E;Martin C
• 通讯作者: Martin C

Manipulation of SlMXl for enhanced carotenoids accumulation and drought resistance in tomato

• DOI: 10.1007/s11434-016-1108-9
• 发表时间: 2016-09-01
• 期刊: SCIENCE BULLETIN
• 影响因子: 18.9
• 作者: Ewas, Mohamed;Gao, Yanqiang;Luo, Jie
• 通讯作者: Luo, Jie