14TSB_SynBio A High Throughput Miniaturised Mass Spectrometry Tool for Profiling Synthetic Design Libraries

14TSB_SynBio 用于分析合成设计文库的高通量小型化质谱工具

• 批准号: BB/M005577/1
• 负责人: Thomas Ellis
• 金额: $ 11.87万
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FM005577%2F1
• 关键词: 14TSB_SynBio; Throughput; Miniaturised; Mass; Spectrometry

Over the last 10 years, the new field of synthetic biology has advanced the existing science of genetic modification by applying principles of engineering; design, simulation and testing. This approach has allowed us to predictably create exciting new technologies by modifying safe microbes with customised DNA to perform new tasks including computation, multi-input environmental sensing and efficient production of medicinal drugs and bioenergy, primarily through metabolic pathway engineering. As our ability to write DNA and engineer microbes accelerates, we are becoming limited by the lack of high-data, high-throughput cell measurement methods available with which to assess the performance of customised strains. The project links foundational synthetic biology and DNA assembly expertise at Imperial College London with microfluidics and mass spectrometry expertise at the industrial partner, Sphere Fluidics Limited, in order to build, test and demonstrate a novel microfluidics-mass spectrometry tool that should allow in-depth measurement of the performance of thousands of engineered microbes per run. The intention of the project is to develop an equipment set-up (interfaced microfluidics and mass spectrometry) and a methodology that together is a platform tool with which to characterise collections of thousands of engineered microbe strains. This tool can then be sold downstream as a service (or as an equipment set-up) for companies and institutions involved in synthetic biology.

在过去的十年里，合成生物学这一新领域通过应用工程学原理推动了现有的基因改造科学的发展。设计、模拟和测试。这种方法使我们能够通过用定制 DNA 修改安全微生物来执行新任务，包括计算、多输入环境传感以及药物和生物能源的高效生产（主要通过代谢途径工程），从而可预测地创造出令人兴奋的新技术。随着我们编写 DNA 和改造微生物的能力不断增强，我们正因缺乏可用于评估定制菌株性能的高数据、高通量细胞测量方法而受到限制。该项目将伦敦帝国理工学院的基础合成生物学和 DNA 组装专业知识与工业合作伙伴 Sphere Fluidics Limited 的微流控和质谱专业知识联系起来，以构建、测试和演示一种新型微流控-质谱工具，该工具应允许深入研究每次运行时测量数千种工程微生物的性能。该项目的目的是开发一种设备设置（微流体和质谱连接）和一种方法，两者共同构成一个平台工具，用于表征数千种工程微生物菌株的集合。然后，该工具可以作为服务（或设备设置）向下游出售给涉及合成生物学的公司和机构。

项目成果

Off-Colony Screening of Biosynthetic Libraries by Rapid Laser-Enabled Mass Spectrometry.

通过快速激光质谱法对生物合成文库进行菌落外筛选。

• DOI: http://dx.10.1021/acssynbio.9b00243
• 发表时间: 2019
• 期刊: ACS synthetic biology
• 影响因子: 4.7
• 作者: Gowers GF