OLFACTORY CORTEX

嗅觉皮层

• 批准号: 3215651
• 负责人: JOSEPH L PRICE
• 金额: $ 16.63万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1976
• 资助国家: 美国
• 起止时间: 1976-09-01 至 1994-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3215651
• 关键词: afferent nerve; association learning; autoradiography; axon; cats; cerebellar cortex; cerebral cortex; cerebral ventricles; cognition; efferent nerve; electron microscopy; electrophysiology; histochemistry /cytochemistry; immunochemistry; laboratory rat; neural information processing; neuroanatomy; neurophysiology; newborn animals; olfactory lobe; thalamus

The structure and functional organization of several interrelated structures of the basal forebrain and anterior temporal lobe will be studied in the rat and monkey. The structures to be studied include the amygdaloid complex, the largely cholinergic nucleus basalis and diagonal band nuclei, the ventral striatum and pallidum, the olfactory and entorhinal cortices, and other cortical areas of the anterior temporal lobe. However, the major emphasis in this grant period will be on the medial, magnocellular part of the mediodorsal thalamic nucleus (MDm) and the orbital and mesial parts of the frontal cortex, which are tightly interconnected with each other by thalamocortical and corticothalamic fibers, and which both receive inputs from the basal forebrain and anterior temporal lobe. All together, these structures form a complex associative system, which has been implicated in memory processing as well as in forebrain control of visceral mechanisms, and is involved in such neurological disorders as epilepsy, autism and Alzheimer's disease. In order to analyze and correlate the connections between these structures, and especially to analyze the afferent fibers to MDm and the functional subdivisions of the orbital and mesial cortex, several anterograde and retrograde axonal tracers will be used. These include 3H-leucine, phaseolus vulgaris leucoagglutinin (PHA-L), WGA-HRP, and the fluorescent dyes "true blue", "fast blue", "diamidino yellow" and "fluorescent gold", as well as the "transmitter specific" tracers 3H-D-aspartate and 3H-GABA. In most cases two or more tracers will be combined, in order to label and compare different fiber systems in a single experiment. In addition, electrophysiological recording of responses to olfactory bulb stimulation will be used to map the olfactory related area of the orbital cortex, and to guide the placement of microinjection of the axonal tracers.

几个相互关联的结构和功能组织 基底前脑和前颞叶的结构 在老鼠和猴子身上进行了研究。 要研究的结构包括 杏仁核复合体，主要是胆碱能基底核和对角核 带核、腹侧纹状体和苍白球、嗅觉和 内嗅皮质和前颞叶的其他皮质区域 叶。 然而，本次资助期间的主要重点将是 丘脑内侧核（MDm）的内侧大细胞部分和 额叶皮层的眶部和内侧部分紧密相连 通过丘脑皮质和皮质丘脑相互连接 纤维，并且都接收来自基底前脑和前脑的输入 颞叶。 这些结构共同构成了一个复杂的关联 系统，它与内存处理以及 前脑控制内脏机制，并参与此类 神经系统疾病，如癫痫、自闭症和阿尔茨海默病。 为了分析和关联这些结构之间的联系， 特别是分析 MDm 的传入纤维和功能 眶皮质和内侧皮质的细分，几个顺行和 将使用逆行轴突示踪剂。 这些包括 3H-亮氨酸、 菜豆白细胞凝集素 (PHA-L)、WGA-HRP 和荧光 染料“真蓝”、“固蓝”、“二脒黄”和“荧光金”， 以及“发射机特异性”示踪剂 3H-D-天冬氨酸和 3H-GABA。 在大多数情况下，将组合两个或多个示踪剂，以便标记和 在一次实验中比较不同的纤维系统。 此外， 对嗅球刺激反应的电生理记录 将用于绘制眼眶皮层嗅觉相关区域的地图，以及 指导轴突示踪剂显微注射的放置。