NOVEL HUMAN ENDOGENOUS RETROVIRUSES

新型人类内源性逆转录病毒

• 批准号: 3185660
• 负责人: ANTHONY J FARAS
• 金额: $ 10.34万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-04-15 至 1990-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3185660
• 关键词: Retroviridae disease; alpharetrovirus; gel electrophoresis; gene expression; genetic manipulation; genetic mapping; germ cells; human tissue; laboratory mouse; laboratory rabbit; molecular cloning; nucleic acid hybridization; nucleic acid probes; nucleic acid sequence; oncogenic virus; provirus; synthetic peptide; tissue /cell culture; viral carcinogenesis; virus DNA; virus genetics; virus protein

The studies proposed in this grant application exemplify a continuation and extension of our ongoing work on the isolation, characterization and expression of new families of endogenous retroviruses. To this end, we plan to isolate and characterize a purportedly novel class of human endogenous retroviruses that we have identified with avian retrovirus-specific genetic probes that we have previously used to identify new classes of avian endogenous retroviruses. Our proposed studies include: (1) isolation of the avian retrovirus-related human endogenous virus family by molecular cloning and characterization by nucleotide sequence analysis; (2) characterization of the extent and nature of expression of this additional family of human endogenous retroviruses in normal and malignant human cell types; and (3) a comparison of this novel endogenous retrovirus family as well as the other previously identified families of human endogenous retroviruses with "C" type virion particles produced by various human tumors including testicular germ cell tumors and differentiating populations of embryonal carcinoma cells to determine whether the "C" type particles produced by these germ cell tumors exhibit any sequence homology to any of the families of human endogenous viruses. The methodologies that we plan to employ to achieve these objectives are, for the most part, already implemented in our laboratory and include a variety of physiochemical, enzymological and hybridization techniques available for the analysis of DNA, RNA, and protein, including nucleotide sequence analysis.

本授予申请中提出的研究举例说明了 继续和扩展我们正在进行的隔离工作， 内生的新家族的表征和表达 逆转录病毒。 为此，我们计划隔离和表征 据称是我们的新型人类内源性逆转录病毒 已经用禽逆转录病毒特异性遗传探针确定了 我们以前曾用来识别新的禽类 内源性逆转录病毒。 我们提出的研究包括：（1） 分离禽逆转录病毒相关的人内源性病毒 通过分子克隆和核苷酸表征的家族 序列分析； （2）表征的程度和性质 这个额外的人内源性家族的表达 正常和恶性的人类细胞类型的逆转录病毒； （3）a 比较这个新型内源性逆转录病毒家族以及 其他先前确定的人内源性家族 用各种产生的“ C”类型病毒颗粒的逆转录病毒 人类肿瘤，包括睾丸生殖细胞肿瘤和 区分胚胎癌细胞种群 确定这些胚芽产生的“ C”类型粒子是否 细胞肿瘤与任何家族都表现出任何序列同源性 人类内源性病毒。 我们计划的方法 实现这些目标的雇用大多数已经是 在我们的实验室中实施，包括各种各样的 理化，酶学和杂交技术 可用于分析DNA，RNA和蛋白质，包括 核苷酸序列分析。