AVIAN LEUKOSIS VIRUSES AND CANCER

禽白血病病毒和癌症

• 批准号: 3167543
• 负责人: HARRIET L ROBINSON
• 金额: $ 24.96万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-01-01 至 1991-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3167543
• 关键词: Retroviridae; Retroviridae disease; alpharetrovirus; autoradiography; avian leukosis virus; cancer risk; chickens; disease /disorder model; gel electrophoresis; gene expression; genetic manipulation; lymphocyte; molecular oncology; neoplasm /cancer genetics; nucleic acid repetitive sequence; oncogenes; osteopetrosis; provirus; radiotracer; tissue /cell culture; transfection; viral leukemogenesis; virus RNA; virus envelope; virus genetics; virus protein

Avian leukosis viruses (ALVs) and in vitro constructed recombinants of ALVs will be used to study the roles of viral and host genes in the induction of cancer. The molecular virology of four different virally induced cancers will be studied: B-cell lymphoma, erythroblastosis, angiosarcoma, and fibrosarcoma. The first specific aim is to define ALV sequences that determine lymphomogenic potential. These studies will focus on the roles of sequences near the 5' long terminal repeat (LTR) and at the 5' end of the env gene in cancer induction. These studies will also examine the role of deletions near the 5' LTR in the upregulation of c-myc transcription by ALV insertions. The second specific aim is to define the role of the C-terminal domain of the epidermal growth factor (EGF) receptor in the normal and malignant growth of erythroid, endothelial, and fibroblastic cells. These studies will define mutations in the C-terminal domain of the EGF receptor that determine the potential for viral-erbB genes to cause specific diseases. Studies will also be the C- terminal domain of the EGF receptor. The third specific aim is to define whether one or more than one gene determines the unusual susceptibility of inbred line 151 chickens to erythroblastosis induction by viral-erbB proteins encoding the complete C- terminus of the EGF receptor.

禽白血病病毒（ALV）和体外构建 ALV的重组将用于研究病毒和 癌症诱导中的宿主基因。 分子病毒学 将研究四种不同病毒诱导的癌症：B细胞 淋巴瘤，红细胞增生，血管肉瘤和纤维肉瘤。 第一个具体目的是定义确定的ALV序列 淋巴发型潜力。 这些研究将侧重于 5'长时间重复（LTR）和5'端附近的序列 癌症诱导中的ENV基因。 这些研究也将 检查5'ltr附近缺失在上调中的作用 通过ALV插入的C-MYC转录。 第二个特定 目的是定义C末端域的作用 正常和表皮生长因子（EGF）受体 红细胞，内皮和成纤维细胞的恶性生长。 这些研究将定义在C末端结构域中的突变 确定病毒-ERBB潜力的EGF受体 引起特定疾病的基因。 研究也将是C- EGF受体的末端结构域。 第三个具体目的是 定义一个或多个基因确定不寻常的基因 近交系列151只鸡对红细胞增生的敏感性 通过编码完整c-的病毒-ERBB蛋白诱导 EGF受体的末端。