Combining field phenotyping and next generation genetics to uncover markers, genes and biology underlying drought tolerance in wheat.

结合田间表型和下一代遗传学，揭示小麦耐旱性背后的标记、基因和生物学。

• 批准号: BB/L011786/1
• 负责人: Anthony Hall
• 金额: $ 97.33万
• 依托单位: University of Liverpool
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FL011786%2F1
• 关键词: Combining; field; phenotyping; next; generation

Food security is internationally recognised as one of the major global challenges of the 21st century. By 2050, it is predicted that world food production will have to increase by 50% to meet demand. This is against the pressures of global climate change and resource limitations. Meeting this challenge is going to require the development of innovative strategies to make use of our unprecedented knowledge of modern bioscience in the post genomic era. Developing new varieties of wheat will be fundamental to meeting the 2050 goal. For wheat growers Internationally and specifically in India one of the key issues is drought. Drought means farms cannot always guarantee a good harvest with major implications for the livelihoods and household food security of small-holder farmers. Low rainfall also reduces the land area that can be farmed. Throughout the last century improvements in drought tolerance have come through breeding crops that grow in such a way that drought and drought sensitive stages of growth do not coincide. There is however, huge potential to breed new varieties capable of maintaining stable yields in drought conditions. In collaboration with the University of Bristol and the John Innes Centre (Norwich), work at the University of Liverpool has generated sequence data for the wheat genome. Using this information we have developed new methods to rapidly uncover the genetic variation in wheat. By combining an understanding of genetic variation with a careful study of performance under drought conditions it becomes possible to associate genetic variation with improved drought tolerance. Using this genetic information "molecular marker" tools cab be built, that can be used to rapidly select for lines that are drought tolerant. It is also possible to stack up multiple markers for different traits. As a consortium of interdisciplinary research scientists from the UK and India we plan to use this approach to identify molecular markers associated with drought tolerance in wheat and lay the foundations of an accelerated breeding program to incorporate drought tolerance into Indian wheat varieties. The approaches we will be using will provide a blueprint for how state-of-art technologies can be applied to important food security issues. Much of the output we generate can be used to identify markers for other traits. It will also result in highly trained researchers in India and the UK capable of applying these new approaches. Through outreach work we aim to engage with other researchers and stakeholders and apply this methodology to other traits in wheat and different crops.

粮食安全在国际上被认为是21世纪的全球主要挑战之一。到2050年，预测世界粮食产量必须增加50％才能满足需求。这违背了全球气候变化和资源限制的压力。应对这一挑战将需要制定创新策略，以利用我们在后基因组时代对现代生物科学的前所未有的知识。开发新的小麦品种将是满足2050年目标的基础。 对于国际，特别是在印度的小麦种植者来说，关键问题之一是干旱。干旱意味着农场不能总是保证良好的收获，对小型农民的生计和家庭粮食安全产生重大影响。低降雨也减少了可以耕种的土地面积。在过去的整个世纪中，干旱耐受性的改善是通过繁殖农作物的生长，以干旱和干旱对生长的敏感阶段不一致。但是，有巨大的潜力培养能够在干旱条件下保持稳定产量的新品种。利物浦大学与布里斯托尔大学和约翰·英恩斯中心（诺里奇）合作，已经为小麦基因组生成了序列数据。使用这些信息，我们开发了新方法来快速揭示小麦的遗传变异。通过将对遗传变异的理解与对干旱条件下的性能的仔细研究相结合，可以将遗传变异与改善的干旱耐受性联系起来。使用这些遗传信息“分子标记”工具驾驶室，可用于快速选择耐旱的线条。也可以为不同特征堆叠多个标记。作为来自英国和印度的跨学科研究科学家的财团，我们计划使用这种方法来识别与小麦干旱耐受性相关的分子标记，并为加速育种计划的基础奠定了将干旱耐受性纳入印度小麦品种的基础。我们将使用的方法将为如何将最先进的技术应用于重要的粮食安全问题提供蓝图。我们生成的大部分输出可用于识别其他特征的标记。这还将导致印度和英国训练有素的研究人员能够采用这些新方法。通过宣传工作，我们旨在与其他研究人员和利益相关者互动，并将这种方法应用于小麦和不同作物的其他特征。

项目成果

Maintenance of Photosynthesis as Leaves Age Improves Whole Plant Water Use Efficiency in an Australian Wheat Cultivar

• DOI: 10.3390/agronomy10081102
• 发表时间: 2020-07
• 期刊: Agronomy
• 作者: Bailey Kretzler;Cristina Rodrigues Gabriel Sales;M. Karády;Elizabete Carmo‐Silva;I. Dodd

A genome-wide survey of DNA methylation in hexaploid wheat.

• DOI: 10.1186/s13059-015-0838-3
• 发表时间: 2015-12-10
• 期刊: Genome biology
• 影响因子: 12.3
• 作者: Gardiner LJ;Quinton-Tulloch M;Olohan L;Price J;Hall N;Hall A
• 通讯作者: Hall A

Barley Hv CIRCADIAN CLOCK ASSOCIATED 1 and Hv PHOTOPERIOD H1 Are Circadian Regulators That Can Affect Circadian Rhythms in Arabidopsis.

• DOI: 10.1371/journal.pone.0127449
• 发表时间: 2015
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Kusakina J;Rutterford Z;Cotter S;Martí MC;Laurie DA;Greenland AJ;Hall A;Webb AA
• 通讯作者: Webb AA

A haplotype map of allohexaploid wheat reveals distinct patterns of selection on homoeologous genomes.

• DOI: 10.1186/s13059-015-0606-4
• 发表时间: 2015-02-26
• 期刊: Genome biology
• 影响因子: 12.3
• 作者: Jordan KW;Wang S;Lun Y;Gardiner LJ;MacLachlan R;Hucl P;Wiebe K;Wong D;Forrest KL;IWGS Consortium;Sharpe AG;Sidebottom CH;Hall N;Toomajian C;Close T;Dubcovsky J;Akhunova A;Talbert L;Bansal UK;Bariana HS;Hayden MJ;Pozniak C;Jeddeloh JA;Hall A;Akhunov E
• 通讯作者: Akhunov E

Mapping-by-sequencing in complex polyploid genomes using genic sequence capture: a case study to map yellow rust resistance in hexaploid wheat.

• DOI: 10.1111/tpj.13204
• 发表时间: 2016-08
• 期刊: The Plant journal : for cell and molecular biology
• 作者: Gardiner LJ;Bansept-Basler P;Olohan L;Joynson R;Brenchley R;Hall N;O'Sullivan DM;Hall A
• 通讯作者: Hall A