STRUCTURAL AND CHEMICAL DYNAMICS OF CELL MOVEMENT

细胞运动的结构和化学动力学

• 批准号: 3156306
• 负责人: D. Lansing Taylor
• 金额: $ 16.68万
• 依托单位: CARNEGIE-MELLON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-07-01 至 1991-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3156306
• 关键词: Dictyostelium; actins; birefringences; calcium; cell membrane; cell motility; chemical structure function; chemotaxis; circular dichroism; cytoplasm; cytoskeletal proteins; cytoskeleton; dichroism; electron microscopy; fluorescence microscopy; fluorescent dye /probe; gel filtration chromatography; histochemistry /cytochemistry; microspectrophotometry; molecular site; muscle contraction; muscle relaxation; myosins; optical polarization; phagocytosis; protoplasm motility; radiotracer; striated muscles

The proposed research is aimed at bridging the gap of knowledge between the biochemistry, ultrastructure and cell physiology of cytoplasmic structure and contractility in non-muscle cells. The experiments take advantage of the developments from the last grant period in identifying actin-binding proteins, in developing quantitative fluoresence micropscopic assays, in proposing a working hypothesis on the relationship between cytoplasmic structure and contraction, and in implementing quantitative imaging techniques for basic questions in cell biology. The proposed experiment will continue the plan of integrating information obtained from motile cell extracts, reconstituted cell models, and from living cells. Changes in free [Ca++], pH, solation of the actin-based cytoskeleton, and contraction will be measured in in vitro models and in living cells. In addition, a critical test of the solation-contraction coupling hypothesis is proposed.

拟议的研究旨在弥合知识的差距 胞质结构的生物化学，超微结构和细胞生理 和非肌肉细胞的收缩力。 实验利用了 从上一个赠款期开始识别肌动蛋白结合的发展 蛋白质，在开发定量荧光微观测定时，在 提出关于细胞质之间关系的工作假设 结构和收缩，以及实施定量成像 细胞生物学基本问题的技术。 提出的实验 将继续集成从通气单元获得的信息的计划 提取物，重建的细胞模型以及活细胞。 变更 免费[Ca ++]，pH，基于肌动蛋白的细胞骨架的求解和收缩 将在体外模型和活细胞中测量。 另外， 提出了求解偶联假设的批判性检验。