PATHOGENESIS OF DIABETIC NEPHROPATHY

糖尿病肾病的发病机制

• 批准号: 3151592
• 负责人: ALFRED F MICHAEL
• 金额: $ 12.38万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-07-01 至 1987-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3151592
• 关键词: basement membrane; biopsy; electron microscopy; gel electrophoresis; human subject; immunofluorescence technique; kidney metabolism; membrane structure; radioimmunoassay; radiotracer; renal glomerulus; renal tubule; vascular smooth muscle

The objective of the proposed research is to understand the mechanism of the highly specific increase in albumin in kidney basement membranes (glomerular basement membrane, tubular basement membrane) observed by immunofluorescence and quantitative immunoassay in diabetic nephropathy. A similar increase is observed in diabetic muscle capillary and sarcolemmal basement membranes. This study will define whether the increased binding of albumin to basement membranes is a consequence of an alteration in albumin or in renal basement membranes induced by the diabetic state. Diabetic and normal albumin, obtained from sera and kidney eluates, will be evaluated for differences in binding to basement membrane fractions as well as for structural changes. In addition, basement membrane fractions from diabetic kidneys will be studied for changes that lead to increased entrapment of serum proteins. The presence of increased amounts of albumin in basement membranes in kidney may play an important role in the pathogenesis of diabetic renal and vascular disease.

拟议研究的目的是了解 肾脏基底膜中白蛋白的高度特异性增加（肾小球 地下膜，管状地下膜）通过免疫荧光和 糖尿病性肾病中的定量免疫测定。 类似的增加是 在糖尿病肌肉毛细血管和肌膜基底膜中观察到。 这 研究将定义白蛋白与地下膜的结合是否增加 是白蛋白或肾脏基底膜改变的结果 由糖尿病状态诱导。 从血清获得的糖尿病和正常白蛋白 和肾脏洗脱液，将评估与地下室结合的差异 膜分数以及结构变化。 另外，地下室 将研究来自糖尿病肾脏的膜分数 增加血清蛋白的夹带。 增加数量的存在 肾脏地下室膜中的白蛋白可能在 糖尿病肾脏和血管疾病的发病机理。

项目成果

Lack of tolerance to donor-strain skin grafts in mice with established islet allografts.

已建立胰岛同种异体移植物的小鼠对供体品系皮肤移植物缺乏耐受性。

• 发表时间: 1987
• 期刊: Transplantation
• 影响因子: 6.2
• 作者: Gores,DF;Sutherland,DE;Platt,JL;Bach,FH
• 通讯作者: Bach,FH