The role of the KRAB zinc-finger protein ZFP57 in the maintenance of the methylation programme in mouse development

KRAB 锌指蛋白 ZFP57 在维持小鼠发育中甲基化程序中的作用

• 批准号: BB/G020930/1
• 负责人: Anne Ferguson-Smith
• 金额: $ 46.68万
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2009
• 资助国家: 英国
• 起止时间: 2009 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FG020930%2F1
• 关键词: role; KRAB; zinc; finger; protein

DNA is packaged inside our cells into bundles called chromosomes. Chromosome packaging is a highly regulated process that ensures that the correct cell makes the correct genes available for proper regulation specific to that cell. We are interested in the factors that regulate DNA packaging into chromosomes and in particular we are interested in the changes that happen to chromosome packaging as stem cells develop into body cells. We have made a mutant where key factors required for proper gene regulation are not placed on the DNA and the chromosomes. These experiments are designed to learn more about this key factor and how it influences the chromosomes in early development.

将DNA包装在我们的细胞中，分成称为染色体的束。染色体包装是一个高度调节的过程，可确保正确的细胞使正确的基因可用于特定于该细胞的适当调节。我们对调节DNA包装成染色体的因素感兴趣，尤其是我们对随着干细胞发展到人体细胞中染色体包装发生的变化感兴趣。我们已经制作了一个突变体，其中适当的基因调节所需的关键因素未放在DNA和染色体上。这些实验旨在了解有关此关键因素及其如何影响早期发育中染色体的更多信息。

项目成果

Genomic properties of variably methylated retrotransposons in mouse.

• DOI: 10.1186/s13100-021-00235-1
• 发表时间: 2021-02-21
• 期刊: Mobile DNA
• 影响因子: 4.9
• 作者: Elmer JL;Hay AD;Kessler NJ;Bertozzi TM;Ainscough EAC;Ferguson-Smith AC
• 通讯作者: Ferguson-Smith AC

Variably methylated retrotransposons are refractory to a range of environmental perturbations.

• DOI: 10.1038/s41588-021-00898-9
• 发表时间: 2021-08
• 期刊: Nature genetics
• 影响因子: 30.8
• 作者: Bertozzi TM;Becker JL;Blake GET;Bansal A;Nguyen DK;Fernandez-Twinn DS;Ozanne SE;Bartolomei MS;Simmons RA;Watson ED;Ferguson-Smith AC
• 通讯作者: Ferguson-Smith AC

KRAB zinc finger protein diversification drives mammalian interindividual methylation variability.

• DOI: 10.1073/pnas.2017053117
• 发表时间: 2020-12-08
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: Bertozzi TM;Elmer JL;Macfarlan TS;Ferguson-Smith AC
• 通讯作者: Ferguson-Smith AC

A spontaneous genetically-induced epiallele at a retrotransposon shapes host genome function

逆转录转座子上自发遗传诱导的表观等位基因塑造宿主基因组功能

• DOI: 10.17863/cam.66297
• 发表时间: 2021
• 作者: Bertozzi T

A spontaneous genetically induced epiallele at a retrotransposon shapes host genome function.

• DOI: 10.7554/elife.65233
• 发表时间: 2021-03-23
• 期刊: eLife
• 影响因子: 7.7
• 作者: Bertozzi TM;Takahashi N;Hanin G;Kazachenka A;Ferguson-Smith AC
• 通讯作者: Ferguson-Smith AC